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G4410 Sigma-Aldrich

Griess reagent (modified)

Synonym: Greiss

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Description

General description

Griess reagent is based on Griess reaction. The components include naphthylethylenediamine dihydrochloride suspended in water and sulphanilamide in phosphoric acid. This reagent reacts with nitrite in samples to form a purple azo product, absorbance of which is measured at 546 nm.

Application

Griess reagent (modified) has been used:
• in the nitrite assay of primary spinal cord glial cells stimulated with supernatant of damaged sensory neuron (SDSN)
• for measuring nitrite/nitrate oxidized from nitric oxide in stimulated chondrocytes
• for measuring nitric oxide (NO) degradation in RAW264.7 macrophage cells

Packaging

10 g in poly bottle

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
When using Product G4410, Griess' reagent (modified), what product should I use to make a nitrite standard curve?
Sodium nitrite (Product No. S2252 or 237213) can be used to make a standard curve. A typical standard curve would cover the range of 0-100 microMolar nitrite.
Will phenol red interfere with the Griess reaction Product G4410, Griess' reagent (modified)?
While phenol red does not interfere with the Griess reaction, it will interfere with the absorption reading taken at 540nm.It is recommended to use media without phenol red to avoid any interference in the absorption readings.
Is there an enzymatic method to convert nitrate to nitrite?
A coupled assay to convert nitrate to nitrite has been published by Grisham, M.B., et al., Quantitation of nitrate and nitrite in extracellular fluids. Methods Enzymol., 268, 237-246 (1996).For this protocol, we recommend using Product No. N7265 (Nitrate Reductase), Product No.L1378 (L-Lactic Dehydrogenase), Product No.N1630 (NADPH), Product No. P8574 (Sodium pyruvate) and Product No. F6625 (Flavin adenine dinucleotide disodium salt).
What is the difference between Product G4410 Griess reagent (modified), and Product 03553, Griess reagent?
There are many formulations of the Griess Reagent.The formulations for these products are similar; both are proprietary.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Protocols & Articles
Peer-Reviewed Papers
15

References

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