• USA Home
  • G4546 - Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit

EMAIL THIS PAGE TO A FRIEND
G4546 Sigma-Aldrich

Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-FLJ45472, Anti-GFAP, Anti-Intermediate filament protein, GFAP Antibody for Flow Cytometry - Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit, Gfap Antibody Flow Cytometry

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology, Antibodies for Neural Stem Cells,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   flow cytometry: 1:200-1:500
  immunohistochemistry: 1:100-1:200
  western blot: 1:500-1:1,000
species reactivity   human, mouse, rat
mol wt   antigen mol wt ~50 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   P14136
Gene Information   human ... GFAP(2670)
mouse ... Gfap(14580)
rat ... Gfap(24387)

Description

General description

Intermediate filaments (IFs) with characteristic 10 nm diameter are a distinct class of molecularly heterogenous cytoskeletal filaments defined by ultrastructural, immunological, and biochemical criteria. Intermediate filaments differ significantly from the other cytoskeletal elements of the cell, namely microtubules and microfilaments, and are components of most eukaryotic cells. Glial Fibrillary Acidic Protein (GFAP) (molecular weight of 50 kDa) is found in astrocytes and ependymal cells of the central nervous system. It is found in various other cell types including stellate cells, chondrocytes, osteocytes, keratinocytes, Leydig cells and fibroblasts. GFAP is involved in mitosis, and cell:cell interactions and communications. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system.

Specificity

Rabbit polyclonal anti-Glial Fibrillary Acidic Protein recognizes human, mouse, and rat GFAPs.

Immunogen

synthetic peptide corresponding to an internal C-terminal sequence of human GFAP.

Application

Rabbit polyclonal anti-GFAP antibody is used to tag glial fibrillary acidic protein for detection and quantitation by Western blotting and immunohistochemical (IHC) techniques. It is used as a probe to determine the roles of glial fibrillary acidic protein in cell:cell communications, mitosis and cell structure. Anti-GFAP has proven a valuable tool for use in immunocytochemical localization of GFAP in normal central nervous system tissue, certain tumors and metastases of the glial antigen, as well as for immunofluorescent labeling of cultured mammalian cells.

Physical form

solution in phosphate buffered saline, containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

antibody

Product #

Description

Add to Cart

G6171 Anti-Glial Fibrillary Acidic Protein antibody, Mouse monoclonal, clone G-A-5, purified from hybridoma cell culture

related product

Product #

Description

Add to Cart

G9269 Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
C9205 Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP)−Cy3 antibody produced in mouse, clone G-A-5, purified from hybridoma cell culture
G3893 Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody produced in mouse, clone G-A-5, ascites fluid

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?