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  • G4915 - Anti-Gαi1,2 antibody produced in rabbit

G4915 Sigma

Anti-Gαi1,2 antibody produced in rabbit

whole antiserum



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to G Proteins, Antibodies to G Proteins and Cyclic Nucleotides,
species reactivity   mammals
application(s)   western blot: 1:100-1:200 using cell membranes
clone   polyclonal
antibody form   whole antiserum
mol wt   antigen mol wt 40 kDa
contains   ≤0.1% sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... GNAI2(2771)
mouse ... Gnai2(14678)
rat ... Gnai2(81664)
biological source   rabbit
conjugate   unconjugated



Synthetic peptide corresponding to the C-terminal region of Gα i1,2.

General description

G-proteins are membrane associated heterotrimeric proteins that are comprised of α-, β-, and γ-subunits. These proteins play a key role in signal transduction pathways that regulate cell′s response to several hormones, neuromodulators and ligands. All α subunits appear to be palmitoylated near the N-terminus. Palmitate regulates GTPase activity by GTPase activating proteins.


Anti-Gαi1,2 antibody produced in rabbit is suitable for use as a primary antibody at a working dilution of 1:1000 in immunocytochemical staining of oocytes of the starfish Asterias amurensis after induction of maturation.

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Biochem/physiol Actions

The gene GNAI2 (guanine nucleotide binding protein (G protein), α inhibiting activity polypeptide 2) encodes an α subunit of guanine nucleotide binding proteins. The α-subunit contains a guanine-binding domain that is in its inactive state when it is occupied by GDP. Upon activation, GDP is replaced with GTP, causing the dissociation of the α-subunit from the βγ-subunit complex. This enables the Gα-GTP complex to bind to and regulate specific signaling pathways. GTP is then hydrolyzed, allowing for re-association of the α-subunit with the βγ-subunit complex. This subunit has the binding site for guanine nucleotide and regulates adenylate cyclase. G αi-2 is localized to the basolateral plasma membrane.Carboxy-terminal end plays a key role in the specificity of membrane targeting in Gα i proteins. When coupled to phospholipase C, the α subunits may have a role in pertussis toxin-insensitive pathways.

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