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G5035 Sigma-Aldrich

Granulocyte-Macrophage Colony-Stimulating Factor human

GM-CSF, recombinant, expressed in E. coli, suitable for cell culture

Synonym: GM-CSF human

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Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines and Growth Factors, Cytokines as adjuvants,
biological source   human
recombinant   expressed in E. coli
assay   ≥98% (SDS-PAGE)
form   lyophilized powder
potency   ≤0.1 ng/mL EC50
mol wt   14.6 kDa
packaging   pkg of 4X5 μg
  pkg of 5 μg
storage condition   avoid repeated freeze/thaw cycles
impurities   ≤1.000 EU/μg
color   white
solubility   water: soluble 0.010 mL, clear, colorless
suitability   suitable for cell culture
UniProt accession no.   P04141
storage temp.   −20°C
Gene Information   human ... CSF2(1437), CSF2(1437)

Description

Analysis Note

The proliferative activity of human GM-CSF is tested in culture using human TF-1 cells.

Biochem/physiol Actions

Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.

General description

Package size based on protein content

Physical form

Lyophilized from a 0.2 μm filtered, 10 mM sodium citrate, pH 3.5 solution.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

How does the storage temperature relate to shipping conditions?
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How many units are present in the vial of Product G5035, Granulocyte-Macrophage Colony-Stimulating Factor human?
The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is less than or equal to 0.1 ng/ml, corresponding to a specific activity of greater than or equal to 1 x 107 units/mg. So the 5 microgram package size (G5035-5UG) should have at least 50,000 units per vial.
There is no visible powder in the vial of Product G5035, Granulocyte-Macrophage Colony-Stimulating Factor human. Is the vial empty?
This product only contains 5 micrograms of protein without any carrier protein. The product is lyophilized from a small amount of solution so the product may not be visible in the vial.
Can I use Product G5035, Granulocyte-Macrophage Colony-Stimulating Factor human, on mouse cells?
There is only about 56% homology between human and mouse GM-CSF, and no cross-species activity.We would recommend to use mouse GM-CSF, Product No. G0282.
What is the isoelectric point (pI) for Product G5035, Granulocyte-Macrophage Colony-Stimulating Factor human?
The pI is 4.8.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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