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G5038 Sigma-Aldrich

Anti-Glutamic Acid Decarboxylase 65 (5-22) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-GAD 65

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins, Bioactives, Markers, and Receptors for Stem Cell Biology, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Neurobiology,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using tissue sections of rat pancreas.
  microarray: suitable
  western blot: 1:4,000 using rat brain extract
species reactivity   human, pig, rat, mouse, monkey
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
antibody product type   primary antibodies
UniProt accession no.   Q05329
Gene Information   human ... GAD2(2572)
mouse ... Gad2(14417)
rat ... Gad2(24380)

Description

General description

GAD is predominantly expressed in the central nervous system (CNS) and pancreatic islets. It is also expressed in testis, oviduct and ovary. GAD exists in two isofoms, GAD 65 and GAD 67. GAD65 is targeted to membranes and nerve endings.

Specificity

Reacts specifically with GAD 65 (65 kDa).

Immunogen

synthetic peptide corresponding to the N-terminal region of human GAD 65 (amino acids 5-22). The sequence is highly conserved in rat and pig GAD 65 (single amino acid substitution) and mouse and monkey GAD 65, but is not found in GAD 67.

Application

Anti-Glutamic Acid Decarboxylase 65 antibody produced in rabbit is suitable for use as a primary antibody at a working dilution of 1:1000 in fluorescent immunohistochemistry of stomach and jejunum sections of mice. It is suitable for immunoblotting at a working dilution of 1: 4000 using a rat brain extract. It is also suitable for indirect peroxidase immunohistochemical staining of formalin-fixed, paraffin-embedded sections of rat pancreatic islet cells at a working dilution of 1:1000.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of L-glutamate to γ-aminobutyric acid (GABA), the principal inhibitory neurotransmitter in the brain, and a putative paracrine signal molecule in pancreatic islets. GAD65 is an ampiphilic, membrane-anchored protein, (585 amino acid residues) and is encoded on human chromosome 10. It has been identified as an autoantigen in insulin-dependent diabetes mellitus (IDDM) and stiff-man syndrome (SMS) and may serve as a marker in the early stages of IDDM. GAD 65 synthesizes transmitter GABA for vesicular release.

Safety & Documentation

Safety Information

WGK Germany 
3

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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