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G5166 Sigma-Aldrich

PNGase F from Elizabethkingia miricola

buffered aqueous solution

Synonym: N-Glycosidase F, Peptide N-glycosidase



General description

PNGase F (Peptide N-glycosidase F) cleaves asparagine-linked glycoproteins, which produces carbohydrate-free peptides and detached full-length oligosaccharides.


PNGase F from Elizabethkingia miricola has been used for the deglycosylation of the proteins.

Used to deglycosylate protein.

Biochem/physiol Actions

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

Unit Definition

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

Physical form

Supplied as a solution in 20 mM Tris HCl, pH 7.5, 50 mM NaCl and 1 mM EDTA

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


N-Linked Glycan Strategies

Use of the endoglycosidic enzymes such as PNGase F (N-Glycosidase F) is the most effective method of removing virtually all N-linked oligosaccharides from glycoproteins. A variety of endoglycosidases...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Detergents, Digestions, Mass spectrometry, Nucleic acid denaturation, Proteomics

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Peer-Reviewed Papers


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