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G5298 Sigma-Aldrich

Glutaredoxin-1 human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym: GLRX1, GRX1, TTase-1, Thioltransferase-1

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Cell Stress, Nitric Oxide and Cell Stress, Oxidative Stress Proteins and Reagents More...
recombinant   expressed in E. coli
assay   ≥90% (SDS-PAGE)
form   lyophilized powder
activity   ≥300 units/mg protein
storage condition   desiccated
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... GLRX(2745)

Description

Biochem/physiol Actions

Glutaredoxins (GRXs) participate in thio-disulfide exchange reactions in the presence of GSH, NADPH, and glutathione reductase. Glutaredoxins and thioredoxins belong to related families of low molecular mass enzymes that catalyze thio-disulfide exchange reactions. These enzymes are involved in electron transport, formation of disulfide linkage, protein folding, and protein regulation by thiol redox control.
Two Glutaredoxins have been identified in mammals:
• GRX1: found in the cytosol, supplies ribonucleotide reductase with electrons and is involved in general disulfide-dithiol exchanges, dehydroascorbate reduction, cellular differentiation, regulation of transcription factors and apoptosis.
• GRX2: plays a role in the cellular response to apoptotic stimuli and oxidative stress at the mitochondrial checkpoint. GRX2 has two isoforms (GRX2a and GRX2b) derived from alternative first exons. GRX2a is targeted to mitochondria, whereas GRX2b is predicted to be localized in the nucleus. Unlike GRX1, GRX2 is not inhibited by oxidation of structural Cys residues. In addition, GRX2 can receive electrons not only from GSH, but also from thioredoxin reductase supporting both monothiol and dithiol reactions.

Unit Definition

1 unit oxidizes 1 μmole of NADPH per minute at pH 8 at 25 °C in a coupled reaction with glutathione reductase.

Physical form

Supplied as lyophilized powder containing Glutaredoxin-1 (2 mg/ml), 10 mM MES buffer, 100 mM NaCl, and 1 mM DTT, 1 mM EDTA.

Reconstitution

Reconstitute with 125 μL of 50% glycerol. The reconstituted product contains 2 mgP/mL in 10 mM MES, pH 6, 100 mM NaCl, 1 mM glutathione, and 50% glycerol.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles
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