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G5422 Sigma-Aldrich

β-Glycerophosphate disodium salt hydrate

≤1.0 mol % L-α-isomer

Synonym: BGP, Glycerol 2-phosphate disodium salt hydrate

  • CAS Number 154804-51-0

  • Linear Formula (HOCH2)2CHOP(O)(ONa)2 · xH2O

  • Molecular Weight 216.04 (anhydrous basis)

  •  EC Number 212-464-3

  •  MDL number MFCD00149082

  •  PubChem Substance ID 329799941

  •  NACRES NA.25

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Properties

Related Categories Biochemicals and Reagents, Carbohydrates, Carbohydrates A to Z, Carbohydrates G, Enzyme Inhibitors,
Quality Level   300
biological source   synthetic
form   powder
impurities   ≤0.1% Inorganic phosphorus
  ≤1.0 mol % L-α-isomer
mp   102-104 °C (lit.)
solubility   water: 50 mg/mL, clear, colorless
SMILES string   O.[Na+].[Na+].OCC(CO)OP([O-])([O-])=O
InChI   1S/C3H9O6P.2Na.H2O/c4-1-3(2-5)9-10(6,7)8;;;/h3-5H,1-2H2,(H2,6,7,8);;;1H2/q;2*+1;/p-2
InChI key   ROPZSVKNEIIIDE-UHFFFAOYSA-L

Description

Application

Initiates differentiation of bone-marrow-derived stem cells embedded in a natural collagen/chitosan gel designed for tissue engineering.

β-Glycerophosphate is a classical serine-threonine phosphatase inhibitor used in kinase reaction buffers. BGP is often used in combination with other phosphatase/protease inhibitors for broad spectrum inhibition. It functions as an organic phosphate donor and has been used in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. BGP is also used to buffer M17 media for Lactococcus culture in recombinant protein expression.

β-glycerophosphate is used in homegenization solutions for muscles extracts following fixation.

Packaging

25, 100, 500 g in poly bottle

Quality

Low inorganic phosphate content. Mixtures of α- and β-glycerophosphate have historically been used in microbiological media but high levels of inorganic (free) phosphate has been associated with the irregular growth of bacteria and unwanted precipitates.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
UA0600000

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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