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G5516 Sigma

Glycerol

for molecular biology, ≥99%

Synonym: 1,2,3-Propanetriol, Glycerin

  • CAS Number 56-81-5

  • Linear Formula HOCH2CH(OH)CH2OH

  • Molecular Weight 92.09

  •  Beilstein Registry Number 635685

  •  EC Number 200-289-5

  •  MDL number MFCD00004722

  •  PubChem Substance ID 24895216

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Properties

Related Categories Biochemicals, Biochemicals and Reagents, Carbohydrates, Carbohydrates A to Z, Carbohydrates G,
grade   for molecular biology
  reagent
vapor density   3.1 (vs air)
vapor pressure   <1 mmHg ( 20 °C)
InChI Key   PEDCQBHIVMGVHV-UHFFFAOYSA-N
assay   ≥99%
autoignition temp.   698 °F
refractive index   n20/D 1.474(lit.)
bp   182 °C/20 mmHg(lit.)
mp   20 °C(lit.)
density   1.25 g/mL(lit.)
cation traces   Fe: ≤5 ppm
  Mg: ≤5 ppm
  heavy metals (as Pb): <5 ppm
foreign activity   DNase, RNase, NICKase, and protease, none detected

Description

Application

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Glycerol has been used as:
• A supplement during cell culture of Mycobacterium tuberculosis and Mycobacterium avium.
• A fuel during designing enzymatic biofuel cell.
• A liquid composite matrix with 4-HCCA and 3-amino­quinoline for analysis of neutral and acidic glycans.
• A matrix for fast atom bombardment MS.
• May be employed as liquid matrix for the quantification studies by MALDI (Matrix-assisted laser desorption/ionization mass spectrometry) analysis.

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Packaging

1, 4 L in poly bottle

100, 500 mL in poly bottle

Biochem/physiol Actions

Glycerol is hygroscopic in nature and is soluble in water owing to its three hydrophilic alcoholic hydroxyl groups. It can form both inter- and intramolecular hydrogen bonds, making it a very flexible molecule. The physiologic effect of glycerine is due to cell-mediated immunity, increased IgG production and increased histamine release.

General description

Glycerol is odourless, colorless, viscous in nature, and exists as a sweet tasting liquid. It can be derived naturally as well as from petrochemical feedstock. Glycerol has a wide variety of applications, and is one of the most valuable and versatile chemical substances in nature. It can be used as an emollient, solvent, sweetening agent, in pharmaceutical formulations, cosmetics, foodstuffs and toiletries. It is very stable and can be easily stored under normal temperature; also it is non-irritating and has no adverse impact to the environment.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
MA8050000
Flash Point(F) 
320 °F
Flash Point(C) 
160 °C

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the viscosity of glycerol?
The viscosity of glycerol is 648 Centistokes at 20 Deg C (68 Deg F).
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Stabilizer Protein and RNA Stabilization Reagents
Protocols & Articles

Articles

Introduction to Western Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is termed as blotting. Proteins resolved on Sodium dodecyl sulfate - polyacrylamide gel electrophor...
Keywords: Buffers, Cell disruption, Detergents, Electroblotting, Electrophoresis, Gel electrophoresis, Homogenization, Immobilization, PAGE, Protein assay, Protein extraction, Sample preparations, Separation, Size-exclusion chromatography, Western blot

Introduction to Yeast Media

Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced. They are relatively easy to grow under laboratory conditions. Moreover, despite thei...
Keywords: Culture media, Fermentation, Gene expression, Indicators, Molecular biology, Sterilizations, Transcription, Vitamins, transformation

MSMLS Online Plate Map

Metabolite descriptors included with the software download upon purchase of the product include: Name, Parent CID, molecular formula, molecular weight, CAS, ChEBI, HMDB ID, PubChem Compound and Subst...
Keywords: Mass spectrometry, Metabolites

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. Sigma® Life Science is an essent...
Keywords: Cell disruption, Gene expression, transformation

Protocols

A general protocol for the generation of Nanobodies for structural biology

Els Pardon a, b , Toon Laeremans a, b , Sarah Triest a, b , Søren G F Rasmussen c , Alexandre Wohlkönig a, b , Armin Ruf d , Serge Muyldermans b, e , Wim G J Hol f , Brian K Kobilka g , Jan Steyaert ...
Keywords: AGE, Adsorption, Amplification, Antibiotics, Biochemistry, Biotinylations, Buffers, Cancer, Centrifugation, Chromatography, Cloning, Coagulation, Crystallization, DNA purification, Deaminations, Degradations, Detergents, Digestions, Diseases, Drug discovery, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Genetic, Genomics, Hematology, High performance liquid chromatography, Immobilization, Immunology, Infectious Diseases, Neuroscience, Nucleic acid annealing, Nucleic acid hybridization, Peptide synthesis, Phase transitions, Polymerase chain reaction, Purification, Sample preparations, Separation, Sequencing, Size-exclusion chromatography, Sterilizations, Transfection, Western blot, transformation

Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis

The transient gene expression system using Arabidopsis mesophyll protoplasts has proven an important and versatile tool for conducting cell-based experiments using molecular, cellular, biochemical, g...
Keywords: Gene expression, Genetic, Genetics, Molecular biology, Transfection

Blue native PAGE

a Molekulare Bioenergetik, Zentrum der Biologischen Chemie, Universitätsklinikum Frankfurt, Theodor-Stern-Kai 7, Haus 26
Keywords: Crystallization, Diffusion, Electroblotting, Isoelectric focusing, Microscopy, PAGE, Scanning electron microscopy, Separation

Calcium Phosphate Transfection Kit Protocol

Calcium phosphate transfection is a commonly used method for the introduction of DNA into eukaryotic cells. This technique has been used to obtain both transient1 and stable2 transfections in a wide ...
Keywords: Antibiotics, Applications, Cell culture, Gene expression, Molecular biology, Transfection, Type

Cell-type specific sequencing of microRNAs from complex animal tissues

Chiara Alberti a , Raphael A Manzenreither b , Ivica Sowemimo b , Thomas R Burkard a, b , Jingkui Wang a , Katharina Mahofsky a , Stefan L Ameres b , Luisa Cochella a
Keywords: AGE, Affinity chromatography, Amplification, Catalysis, Cell disruption, Cell signaling, Centrifugation, Chromatography, Cloning, Electrophoresis, Eliminations, Enzyme activity, Gel electrophoresis, Gene expression, Genetics, Immobilization, Immunoprecipitation, Individual protein Immunoprecipitation, Metabolism, Methylations, Microscopy, Northern blot, Nucleic acid hybridization, Oxidations, PAGE, Peptide synthesis, Polymerase chain reaction, Precipitation, Protein assay, Purification, Recombination, Sequencing, Titrations, Transcription, Western blot

Sample Preparation for Western Blotting: Cell Lysis and Protein Extraction

All the steps for protein extraction from cells or tissue (fresh or frozen) must be carried out at 2-8 °C. The following is the composition of one common lysis buffer that is used to prepare protein ...
Keywords: BCA assay, Cell disruption, Electrophoresis, Gel electrophoresis, Homogenization, Protein assay, Protein extraction

Transforming E. coli with Engineered Plasmid

Inoue and colleagues developed this method in 1990. It works well for many strains commonly used in cloning. The original method calls for growing the overnight E. coli cultures at 18°C. We find that...
Keywords: Antibiotics, Centrifugation, Cloning, Peptide synthesis, Phase transitions, transformation

Tricine–SDS-PAGE

a Molekulare Bioenergetik, Zentrum der Biologischen Chemie, Universitätsklinikum Frankfurt, Theodor-Stern-Kai 7, Haus 26
Keywords: Buffers, Chromatography, Crystallization, Degradations, Detergents, Edman degradation, Electroblotting, Electrophoresis, Gel electrophoresis, PAGE, Phosphorylations, Polymerization reactions, Proteomics, Purification, Respiratory, SDS PAGE, Sample preparations, Separation, Sequencing, Solvents, Western blot

Yeast Growth Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Moreover, replica plating and mutant isolation of yeast cells can be done with relativ...
Keywords: Culture media, Detergents, Genetic, transformation

Peer-Reviewed Papers
15

References

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