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G5545 Sigma-Aldrich

Anti-β-Glucuronidase (C-Terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-GUS

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Properties

Related Categories Alphabetical Index, Antibodies, GD-GL, Plant Biology, Primary Antibodies More...
biological source   rabbit
antibody form   affinity isolated antibody
antibody product type   primary antibodies
clone   polyclonal
form   buffered aqueous solution
mol wt   antigen mol wt 60 kDa
species reactivity   plant
concentration   ~1.5 mg/mL
application(s)   western blot: 1-2 μg/mL using purified GUS from E. coli
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The highly purified anti-ß-glucuronidase rabbit antibody can be used for Western blot of transformed plant tissue. The Escherichia coli ß-glucuronidase (GUS) gene is commonly employed as a reporter gene in plant transformations.

Immunogen

synthetic peptide corresponding to amino acids 589-603 at the C-terminus of E. coli GUS, conjugated to KLH.

Preparation Note

The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

Specificity

Anit-β-Glucuronidase (C-Terminal) recognizes bacterial GUS expressed in transgenic tobacco plants.

The antibody recognizes bacterial GUS expressed in transgenic tobacco plants.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Detection of GUS by immunoblotting (60 kDa). Staining of the GUS band in immunoblotting is specifically inhibited by the immunizing GUS peptide (E. coli, amino acids 589-603).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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G5420 Anti-β-Glucuronidase (N-Terminal) antibody produced in rabbit, ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

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