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G5768 Sigma-Aldrich

Gamborg’s B-5 Basal Salt Mixture

fine powder, suitable for plant cell culture

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Properties

Related Categories Culture Media, Salts and Vitamin Mixes, Molecular Biology, Plant Biotechnology, Plant Tissue Culture, Protoplast Isolation and Culture More...
Quality Level   200
form   fine powder
application(s)   cell culture | plant: suitable
Featured Industry   Agriculture
storage temp.   2-8°C

Description

General description

Gamborg’ B-5 medium described by Gamborg, et al. may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg’s B-5 (B5) medium for in vitro plant culture of cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis.

Application

Gamborg’s B-5 medium has been used for the culture of Annona squamosa callus.

Gamborg′s B-5 Basal Salt Mixture has been used:
• as a constituent during the preparation of TEX buffer
• as a media to maintain potato suspension and callus cultures
• as a media in the primary-node transformation method

Formula variant

With the concentrations of macro- and micronutrients as described by Gamborg, et al. (1968).

Media Formulation

Quantity

Formulated to contain 3.1 grams of powder per liter of medium.

Safety & Documentation

Safety Information

Symbol 
GHS03  GHS03
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
UN 1486 5.1 / PGIII
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

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Protocols & Articles

Protocols

Classical Plant Media Formulations

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today. Below, find formulat...
Keywords: Culture media, Mass spectrometry

Peer-Reviewed Papers
15

References

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