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  • G5796 - Monoclonal Anti-Gemin 8 antibody produced in mouse

G5796 Sigma-Aldrich

Monoclonal Anti-Gemin 8 antibody produced in mouse

~1 mg/mL, clone 1F8, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-Gem associated protein 8



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Epigenetics and Nuclear Signaling, Antibodies for Gene Regulation,
conjugate   unconjugated
clone   1F8, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
  indirect ELISA: suitable
  western blot: 1-2 μg/mL using HeLa nuclear cell extract
species reactivity   human, rat, mouse, canine
mol wt   antigen mol wt ~28 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2a
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   Q9NWZ8
Gene Information   human ... GEMIN8(54960)
mouse ... Gemin8(237221)
rat ... Gemin8(363462)



Gemin 8 recombinant human protein.


Monoclonal Anti-Gemin 8 antibody produced in mouse is suitable for immunocytochemistry, immunoprecipitation and indirect ELISA. It is also suitable for western blot analysis at a working concentration of 1-2μg/mL using HeLa nuclear cell extract.

Target description

GEMIN8 encodes a protein that forms a part of the SMN (Survival of Motor Neurons) complex. The complex is involved in spliceosomal snRNP assembly in the cytoplasm and pre-mRNA splicing and transcription in the nucleus. SMN forms a multi-protein complex with Gemin 2-7 proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

GEMIN8 is integral for the proper structural organization of the SMN complex. It also forms a heteromeric complex with Gemin6, Gemin7 and Unrip and mediates recruitment of Sm protein to the snRNP pathway.

Safety & Documentation

Safety Information

NONH for all modes of transport
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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