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G5893 Sigma-Aldrich

Gamborg’s B-5 Basal Medium with Minimal Organics

fine powder, suitable for plant cell culture

  •  NACRES NA.72

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Properties

Related Categories Culture Media, Salts and Vitamin Mixes, Molecular Biology, Plant Biotechnology, Plant Tissue Culture
Quality Level   200
form   fine powder
application(s)   cell culture | plant: suitable
Featured Industry   Agriculture
storage temp.   2-8°C

Description

General description

Gamborg′s B-5 basal medium is an efficient nutrient mixture for plants.

Application

Gamborg’ B-5 medium may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg’s B-5 (B5) medium for in vitro plant culture of cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis. Gamborg’s B-5 medium has been used for the culture of Annona squamosa callus.

Gamborg′s B-5 basal medium with minimal organics has been used in culturing plant cells of Daucus carota. It has also been used for selection of primary transformants of Arabidopsis thaliana.

Formula variant

With the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1968).

Media Formulation

Quantity

Formulated to contain 3.2 grams of powder per liter of medium.

Safety & Documentation

Safety Information

Symbol 
GHS03  GHS03
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
UN 1486 5.1 / PGIII
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

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Protocols & Articles

Protocols

Classical Plant Media Formulations

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Peer-Reviewed Papers
15

References

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