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  • G6670 - Anti-Growth Factor Independence-1 antibody, Mouse monoclonal

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G6670 Sigma-Aldrich

Anti-Growth Factor Independence-1 antibody, Mouse monoclonal

clone 2.5D.17, purified from hybridoma cell culture

Synonym: Anti-GFI1, Anti-ZNF163

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Epigenetics and Nuclear Signaling, Antibodies for Gene Regulation,
conjugate   unconjugated
clone   2.5D.17, monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable
  microarray: suitable
  western blot: 1-2 μg/mL using total cell extract of A549 cells
species reactivity   mouse, rat, human
mol wt   antigen ~55 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified from hybridoma cell culture
isotype   IgG1
antibody product type   primary antibodies
concentration   ~2 mg/mL
UniProt accession no.   Q99684
Gene Information   human ... GFI1(2672)
mouse ... Gfi1(14581)
rat ... Gfi1(24388)

Description

Specificity

The antibody epitope resides within the GFI1 SNAG transcriptional-repression domain (amino acids 7-26).

Immunogen

rat growth factor independence-1 (GFI1).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

The antibody may also be used in EMSA (electrophoretic mobility shift analysis).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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