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G6751 Sigma-Aldrich

α-Glycerophosphate Dehydrogenase from rabbit muscle

Type I, ammonium sulfate suspension, 100-300 units/mg protein

Synonym: sn-Glycerol-3-phosphate:NAD+ 2-oxidoreductase, sn-Glycerol-3-phosphate Dehydrogenase from rabbit muscle

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Properties

Related Categories 1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
Quality Level   200
type   Type I
form   ammonium sulfate suspension
foreign activity   Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%
  Triosephosphate isomerase ≤0.02%
storage temp.   2-8°C

Description

Unit Definition

One unit will convert 1.0 μmole of dihydroxyacetone phosphate to α-glycerophosphate per min at pH 7.4 at 25 °C.

Physical form

Crystalline suspension in 3.2 M (NH4)2SO4 and 0.1 g/L EDTA solution, pH 6.0

Preparation Note

Prepared by modification of the method of Fondy.

Analysis Note

Protein determined by biuret.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

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Peer-Reviewed Papers
15

References

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