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G6784 Sigma-Aldrich

L-Glutamine–Penicillin–Streptomycin solution

L-glutamine 200 mM, penicillin 10,000 units, streptomycin 10 mg/mL, sterile-filtered, BioReagent, suitable for cell culture

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Properties

Related Categories A - K, Antibacterial, Antibiotic Solutions, Antibiotics, Antibiotics A to Z,
biological source   synthetic
description   stabilized
sterility   sterile-filtered
product line   BioReagent
form   liquid
composition   L-glutamine, 200 mM
  penicillin, 10,000 units
  streptomycin, 10 mg/mL
application(s)   cell culture | mammalian: suitable
impurities   endotoxin, tested
suitability   suitable for cell culture
Mode of action   cell wall synthesis | interferes
  protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
storage temp.   −20°C

Description

Components

This solution is formulated to contain 200 mM L-glutamine, 10,000 units of Penicillin and 10 mg/mL of streptomycin, solubilized in a proprietary buffer.

Application

This antibiotic solution is used to reduce or eliminate bacterial contamination in primary cell cultures. It is recommended for us at 10 mL/L.

Biochem/physiol Actions

Mode of Action: The product acts in three ways: Glutamine is an essential energy source for most mammalian cells in culture due to its amino acid carbon skeleton. Penicillin inhibits bacterial cell wall synthesis. Streptomycin inhibits prokaryote protein synthesis by preventing the transition from initiation complex to chain-elongating ribosome, causing miscoding.

Antimicrobial spectrum: This product acts against both gram-negative and gram-positive bacteria.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin


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Cell Culture Guide
Protocols & Articles

Articles

A functional assay for microRNA target identification and validation

MicroRNAs (miRNA) are a class of small RNA molecules that regulate numerous critical cellular processes and bind to partially complementary sequences resulting in down-regulation of their target gene...
Joop Gäken, Azim M. Mohamedali, Jie Jiang, Farooq Malik, Doris Stangl, Alexander E. Smith, Constantinos Chronis, Austin G. Kulasekararaj, Nicolas S. B. Thomas, Farzin farzaneh, Mahavash Tavassoli, and Ghulam J. Maufi
Nucleic Acids Research 2012, 1-9
Keywords: Cellular processes, Sequences, Western blot

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Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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