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G6880 Sigma-Aldrich

α-Glycerophosphate Dehydrogenase from rabbit muscle

Type X, lyophilized powder, ≥100 units/mg protein

Synonym: sn-Glycerol-3-phosphate:NAD+ 2-oxidoreductase, sn-Glycerol-3-phosphate Dehydrogenase from rabbit muscle

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Properties

Related Categories 1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
Quality Level   200
type   Type X
form   lyophilized powder
composition   Protein, ≥75%
foreign activity   Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%
  Triosephosphate isomerase ≤0.02%
shipped in   wet ice
storage temp.   −20°C

Description

Application

α-glycerophosphate dehydrogenase was used in 2-deoxy-ribose 5-phosphate aldolase (DERA) cleavage (retroaldol) assay.

Biochem/physiol Actions

α-glycerophosphate dehydrogenase catalyzes the conversion of dihydroxyacetone to glycerol phosphate.

Unit Definition

One unit will convert 1.0 μmole of dihydroxyacetone phosphate to α-glycerophosphate per min at pH 7.4 at 25 °C.

Physical form

Lyophilized sulfate-free powder containing buffer salts as citrate and EDTA

Analysis Note

Protein determined by biuret

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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