• USA Home
  • G7017 - β-Glucuronidase from Helix pomatia

EMAIL THIS PAGE TO A FRIEND
G7017 Sigma-Aldrich

β-Glucuronidase from Helix pomatia

Type HP-2, aqueous solution, ≥100,000 units/mL

Synonym: β-D-Glucuronide glucuronosohydrolase

Purchase

Description

General description

β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Application

β-glucuronidase was used in enzymic hydrolysis of tissue homogenates for liquid chromatography-electrospray ion trap mass spectrometry (LC/MSn) analysis, to study the structures of degradation products of baicalin.

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Packaging

25 mL in glass bottle

Biochem/physiol Actions

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Quality

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Linkage

Similar to G0876, but produced by Sigma.

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

Physical form

Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Single- & multi-channel pipettes from BRAND
Protocols & Articles

Articles

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS. Here we evaluate the hydrolysis efficiency of β-gluc...
Jim Blasberg
Harkewal Singh, Kevin Ray Sigma-Aldrich, St. Louis, MO
Keywords: High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites

Protocols

Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver

This procedure applies to all b-Glucuronidase products that are derived from Helix Pomatia and Bovine Liver.
Keywords: Enzymology

Related Content

Analytical Enzyme Beta Glucuronidase

Our β-Glucuronidases are routinely used for the enzymatic hydrolysis of glucuronides from urine1,2, plasma3,4, and other fluids5 prior to analysis by enzyme immunoassay, mass spectrometry, gas chroma...
Keywords: Bacterial conjugations, Cancer, Chromatography, Gas chromatography, High performance liquid chromatography, Immunoassay, Mass spectrometry, PAGE, Purification

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Life Science Materials

With a broad portfolio of widely-cited reagents and kits supported by industry-leading expertise, we help you get the most out of your life science research.
Keywords: Analytical Chemistry, Materials Science

Peer-Reviewed Papers
15

References

Related Products

also commonly purchased with this product

Product #

Description

Add to Cart

P4411 pH test strips, pH 7.0-14.0, resolution: 0.5 pH unit
257478 2,2,3,3,3-Pentafluoro-1-propanol, 97%
T6381 N,O-Bis(trimethylsilyl)trifluoroacetamide with trimethylchlorosilane, with 1% trimethylchlorosilane, for GC derivatization, LiChropur
G0876 β-Glucuronidase from Helix pomatia, Type H-2, aqueous solution, ≥85,000 units/mL
I0880 Isoxsuprine hydrochloride, analytical standard

substrate

Product #

Description

Add to Cart

73677 4-Nitrophenyl β-D-glucuronide, ≥99.0% (TLC)
B8174 5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt, tablet
B4532 5-Bromo-6-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, ≥98%

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?