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G7652 Sigma

Anti-Mouse IgG (whole molecule)–Gold antibody produced in goat

affinity isolated antibody, aqueous glycerol suspension, 10 nm (colloidal gold)



Related Categories Alphabetical Index, Antibodies, Gold, MO-MR, Mouse Secondary Antibodies and Conjugates,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   aqueous glycerol suspension
availability   not available in Canada (at this time; for questions or status updates, please email us at antibody.canada@sial.com)
particle size   10 nm (colloidal gold)
conjugate   gold conjugate
storage temp.   2-8°C



Mouse IgG purified from normal mouse serum


0.4 mL in glass insert

2 mL in glass bottle

Physical form

Colloidal suspension in Tris buffered saline, pH 8.2, with 30% glycerol (v/v), 1% bovine serum albumin (w/v), and 15 mM sodium azide.


Anti-Mouse IgG (whole molecule)-Gold antibody is suitable for use in inductively coupled plasma mass spectrometry (ICPMS)-Linked DNA Assay ((1:100). The product can also be used for immunoelectron microscopy (1:20).

Goat polyclonal anti-mouse IgG (whole molecule)–Gold antibody may be used as a primary or secondary antibody that enables the labeling of mouse IgG for detection by electron microscopy.

General description

Binding activity is determined using a modification of the dot blot assay of Brada and Roth. In this assay a 1 mg/mL solution of mouse IgG is serially diluted in phosphate buffered saline, 1 mL of each dilution is then applied to nitrocellulose paper. Binding activity is defined as the minimum amount of mouse IgG detectable as a visible pink-red spot after a 1 hour incubation with the gold conjugate diluted to an A520 of0.25. Relative order of singlets, doublets, triplets or greater: Singlets>Doublets>Triplets. Clustering data determined by floating the electron microscopy (EM) nickel grid on conjugate diluted 1:100 in Tris buffer, pH 8.0, containing 1% BSA, for 30-60 minutes. The grid is evaluated by EM after drying. One hundred particles are scanned to determine clustering.

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (whole molecule)-Gold antibody is specific for mouse IgG.

Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Immunogold labeling is a technique whereing antibodies, especially secondary antibodies, are labeled with colloidal gold. These gold conjugated antibodies generate electron dense particles that are visable using electron microscopy.

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Safety Information

NONH for all modes of transport
WGK Germany 


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