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G7663 Sigma-Aldrich

Gelatin blocking buffer

for Western blotting, powder blend

Purchase

Description

Application

Suitable for use as a non-specific blocking agent for Western blots.

General description

Gelatin Blocking Buffer is a standard reagent in Western blotting procedures. It is used to block non-specific binding on the membrane, when using nylon or nitrocellulose (but not PVDF).

Packaging

1 L in poly bottle

Reconstitution

Dissolve entire contents of the bottle in a final volume of 1L molecular biology grade water. The resulting solution will be faint yellow in color and does not require filtering or heating before use.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Intro to Southern & Northern Blotting | Northern & Southern Blot Protocols

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Nucleic Acid Electrophoresis Protocols & Introduction

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Protocols

Gelatin Blocking Buffer for Direct Detection Southern or Western Blotting

Product Description Reagents Reagents Required but not Provided Preparation Instructions Storage/Stability Procedure Troubleshooting Guide Materials References
Keywords: Detergents, Enzyme activity, Immobilization, Mass spectrometry, PAGE, Southern blot, Western blot

Peer-Reviewed Papers
15

References

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