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G8043 Sigma-Aldrich

Anti-phospho-Growth Associated Protein-43 (pSer41) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-phospho-GAP-43 (pSer41)

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 1:1,000
species reactivity   rat, human, mouse
mol wt   antigen 43 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   P17677
Gene Information   human ... GAP43(2596)
mouse ... Gap43(14432)
rat ... Gap43(29423)

Description

Immunogen

synthetic phosphopeptide derived from the region of rat GAP-43 phosphorylated on serine 41.

Application

Anti-phospho-Growth Associated Protein-43 (pSer41) antibody produced in rabbit is suitable for immunoblotting at a working dilution of 1:1000.

Physical form

Solution in 10 mM HEPES, pH 7.5, containing 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

GAP-43 is an intracellular phosphoprotein that is present exclusively in the peripheral and central nervous systems. Protein kinase C (PKC) phosphorylates GAP-43 on a single site, serine 41. This phosphorylated protein is found in the areas where growth cones make productive and stable contacts with other cells. It facilitates stabilization of long actin filaments. Phosphorylation by PKC at serine 41 is related to nerve-terminal sprouting and long-term potentiation as high levels of GAP-43 are present in neocortical areas and in the limbic system. It is crucial in mediating experience dependent plasticity.

Analysis Note

An additional band at 150-160 kDa may be detected and reflects the oligomeric nature of GAP-43.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

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Certificate of Origin

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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