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G8168 Sigma-Aldrich

G 418 disulfate salt solution

50 mg/mL in H2O, sterile-filtered, BioReagent, suitable for cell culture

Synonym: Antibiotic G418

  • CAS Number 108321-42-2

  • Empirical Formula (Hill Notation) C20H40N4O10 · 2H2SO4

  • Molecular Weight 692.71

  •  MDL number MFCD00058314

  •  PubChem Substance ID 24895333

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Properties

Related Categories A - K, Aminoglycosides, Antibacterial, Antibiotics, Antibiotics A to Z,
biological source   Micromonospora echinospora
InChI Key   UHEPSJJJMTWUCP-NKCAIAFTSA-N
sterility   sterile-filtered
product line   BioReagent
form   solution
potency   50-60 mg per mL
mol wt   692.7
packaging   pkg of 10 and 100 mL
concentration   50 mg/mL in H2O
impurities   endotoxin, tested
color   colorless
solubility   H2O: 50 mg/mL, clear
suitability   suitable for cell culture
Mode of action   protein synthesis | interferes
antibiotic activity spectrum   parasites
storage temp.   2-8°C

Description

General description

Chemical structure: aminoglycoside

Application

G418 disulfate is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.

Biochem/physiol Actions

Mode of Action: G418 blocks polypeptide synthesis and protein elongation by inhibiting synthesis at the 70S and 80S ribosomes.

Antimicrobial Spectrum: G418 selects for cells stably transfected with an iNOS promoter construct and neomycin resistance gene and shows activity against protozoa and helminthes.

Caution

The product is stable for two years when stored at 2-8°C.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

Articles

Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic to determine the minimum concentration of an antibiotic that c...
Keywords: Antibiotics, Cell culture, Evaporation, Transfection

Safe Handling of Lentivirus

Our lentiviral vector systems have been developed with enhanced safety features. Numerous precautions are in place in the design of our lentiviruses to prevent replication, including eliminating viru...
Keywords: Environmental, Genetic, Recombination, Transfection

Protocols

CRISPR Lentiviral Screening: Antibiotic Selection

A kill curve is a dose-response experiment where cells are subjected to a range of antibiotic concentrations to determine the minimum concentration of antibiotic needed to kill all cells over a desir...
Keywords: Antibiotics, Apoptosis, Gene expression, Transduction

Protocol, FACS Titration of Lentivirus

FACS Analysis of CFU FACS (Fluorescence-Activated Cell Sorting) provides a method for sorting a mixed population of cells into two or more groups, one cell at a time, based on the specific light scat...
Keywords: Enzyme-linked immunosorbent assay, Flow cytometry, Gene expression, Titrations, Transduction

Successful Transduction Using Lentivirus

Successful targeting relies on optimizing key sensitive steps in the process, including lentiviral transduction. Below are some helpful handling and titration tips from our R&D lentiviral experts. As...
Keywords: Antibiotics, Enzyme-linked immunosorbent assay, Gene expression, PAGE, Polymerase chain reaction, Titrations, Transduction

Related Content

Antibiotics Selection Guide

The Antibiotic Selection Guide enables researchers to spend more time on their research discoveries and less time seeking established antibiotic information. Link to hundreds of antibiotics for conta...
Keywords: Antibiotics, Cell biology, Cell culture, Genetic, High performance liquid chromatography, Reversed-phase chromatography

Genome-wide Screen for miRNA Targets Using the MISSION® Target ID Library [VIDEO]

The MISSION Target ID Library is designed to assist in discovery and identification of microRNA (miRNA) targets. The MISSION Target ID Library is a plasmid-based, genome-wide cDNA library cloned into...
Keywords: PAGE, Sequencing

Peer-Reviewed Papers
15

References

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