G8415 Sigma-Aldrich

L-Glutamic acid

from non-animal source, meets EP testing specifications, suitable for cell culture, 98.5-100.5%

Synonym: (S)-2-Aminopentanedioic acid, Glu

  • CAS Number 56-86-0

  • Linear Formula HO2CCH2CH2CH(NH2)CO2H

  • Molecular Weight 147.13

  •  Beilstein/REAXYS Number 1723801

  •  EC Number 200-293-7

  •  MDL number MFCD00002634

  •  eCl@ss 32160406

  •  PubChem Substance ID 24895340

  •  NACRES NA.26



Related Categories Amino Acids, Amino Acids & Vitamins, Amino Acids and Vitamins, Analytical/Chromatography, Asymmetric Synthesis,
Quality Level   200
biological source   non-animal source
Agency/Method   meets EP testing specifications
assay   98.5-100.5%
form   powder
application(s)   cell culture | mammalian: suitable
impurities   endotoxin, tested
color   white
mp   205 °C (dec.) (lit.)
solubility   1 M HCl: 100 mg/mL
density   1.54 g/cm3 at 20 °C
anion traces   chloride (Cl-): ≤200 ppm
  sulfate (SO42-): ≤200 ppm
cation traces   As: ≤1 ppm, passes test
  Fe: ≤10 ppm, passes test
  NH4+: ≤200 ppm, passes test
Featured Industry   Pharmaceutical (small molecule)
storage temp.   room temp
SMILES string   N[C@@H](CCC(O)=O)C(O)=O
InChI   1S/C5H9NO4/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H,7,8)(H,9,10)/t3-/m0/s1
Gene Information   human ... CCR2(1231), GRIA1(2890), GRIA2(2891), GRIA4(2893), GRIK1(2897), GRIK2(2898), GRIK3(2899), GRIK5(2901), GRIN2B(2904), GRM2(2912), SLC1A1(6505), SLC1A2(6506)
rat ... Gria1(50592), Grik1(29559), Grik2(54257), Grik4(24406), Grin2a(24409), Grm1(24414), Grm2(24415), Grm3(24416), Grm4(24417), Grm5(24418), Grm6(24419), Grm7(81672), Slc1a2(29482)



L-Glutamic acid has been used as a supplement in for cell culture media to culture hippocampal neurons and hT-PA (human tissue plasminogen activation) producing cell line.


1 kg in poly bottle

10 mg in glass bottle

100 g in poly bottle

Biochem/physiol Actions

Glutamic acid, or glutamate, the salt form of glutamic acid, functions as a neurotransmitter and also serves as a precursor to other neurotransmitters such as γ-aminobutyric acid. Glutamic acid also plays a key role in many metabolic pathways, that controls growth, reproduction, maintenance and immunity. It is converted to α-ketoglutarate, a key component of the TCA (tricarboxylic acid) cycle, and a precursor for the biosynthesis of nucleic acids and certain amino acids. In cells, glutamine is converted to glutamate by the enzyme glutaminase. Glutamine serves as a source of energy for rapidly dividing cells comprising lymphocytes, enterocytes, macrophages and tumors. Glutamine mediates protein turnover via cellular mTOR (mammalian target of rapamycin) signaling. It is also known to be associated with the inhibition of apoptosis.

An excitatory amino acid neurotransmitter that is an agonist at all subtypes of glutamate receptors (metabotropic, kainate, NMDA, and AMPA).

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Glutamine Metabolism is Dysregulated in Many Cancer Cells

Proliferatively active cells require both a source of carbon and of nitrogen for the synthesis of macromolecules. Although a large proportion of tumor cells utilize aerobic glycolysis and shunt metab...
BioFiles v7 n4
Keywords: Aerobic, Cancer, Citric Acid Cycle, Gene expression, Glycolysis, Metabolism, Metabolites, Phosphorylations, Transcription

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Peer-Reviewed Papers


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