• USA Home
  • G8529 - Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

G8529 Sigma-Aldrich

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

recombinant, expressed in E. coli, lyophilized powder, ≥550 units/mg protein (biuret)

Synonym: G-6-P-DH



Related Categories 1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Carbohydrate Metabolism, Cell Biology,
Quality Level   300
recombinant   expressed in E. coli
form   lyophilized powder
composition   Protein, 10-40% biuret
Featured Industry   Agriculture
foreign activity   creatine phosphokinase, glutathione reductase, myokinase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, hexokinase ≤0.01%
storage temp.   2-8°C



200, 1000 units in glass bottle

2000, 10000 units in poly bottle

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Unit Definition

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.

Physical form

Lyophilized powder containing Ficoll and Tris buffer salts

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
For Product No. G8529, Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides, can the Ficoll present in the product be removed?
Ficoll is added to the enzyme preparation as a stabilizer. The enzyme may become unstable if the Ficoll is removed (i.e., through a dialysis step).
In which solvent can Product No. G8529, Glucose- 6-phosphate Dehydrogenase from Leuconostoc mesenteroides, be dissolved?
The product can be dissolved at 1 mg/ml in 5 mM glycine buffer, pH 8, (with or without 0.1% BSA) or in deionized water at 1 mg/ml (buffer is preferable)
Can solutions of Product No. G8529, Glucose-6-phosphate Dehydrogenase, recombinant from Leuconostoc mesenteroides, be stored?
Sigma has not evaluated the solution stability of this enzyme.Perhaps solutions in buffer may be aliquotted and stored frozen at 20°C for about 1-2 months.Sigma offers the enzyme in an ammonium sulfate suspension, product G8404, for solution storage at 2-8°C.
What are the differences between Product No. G8529, Glucose-6-phosphate Dehydrogenase and Product No. G5885, Glucose-6-phosphate Dehydrogenase?
Product No. G8529 is a recombinant protein from Leuconostoc mesenteroides, expressed in Escherichia coli.Product No. G5885 is a non-recombinant protein from Leuconostoc mesenteroides.
What is the molecular weight of Product No. G8529, Glucose-6-phosphate Dehydrogenase?
Sigma has not determined the molecular weight of this protein, but a value of 55,000 daltons has been reported for the monomer molecular weight (the enzyme from Leuconostoc mesenteroides is reportedly a dimer protein) (Biochem. Biophys. Res. Comm. 59, 894 (1974)).
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions
Protocols & Articles


Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC

To standardize a procedure for the enzymatic determination of Glucose-6-Phosphate Dehydrogenase.
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Committed to developing and providing breakthrough problem-solving reagents and solutions, explore our comprehensive offering of enzymes and protein reagents and kits with the confidence that you wil...
Keywords: Cell signaling, Diagnostic, Enzyme-linked immunosorbent assay, Immunohistochemistry, Metabolism, Molecular biology, Purification, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?