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  • G9152 - Anti-Galactocerebroside antibody produced in rabbit

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G9152 Sigma-Aldrich

Anti-Galactocerebroside antibody produced in rabbit

whole antiserum

Synonym: Galactocerebroside Antibody - Anti-Galactocerebroside antibody produced in rabbit, Galactocerebroside Antibody

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins, Bioactives, Markers, and Receptors for Stem Cell Biology, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   dot blot: 1:50 using galactocerebroside I and II
species reactivity   human
shipped in   dry ice
storage temp.   −20°C
antibody form   whole antiserum
antibody product type   primary antibodies
contains   15 mM sodium azide
UniProt accession no.   P54803
Gene Information   human ... GALC(2581)

Description

Specificity

The antibody localizes oligodendroglia in cultured cells. It may be used for studying changes in the organization of oligodendroglial membrane sheets in culture. Specificity of the product is determined by dot blotting using galactocerebroside as the antigen.

Immunogen

Galactocerebrosides (GalC) I and II conjugated to KLH

Application

Anti-Galactocerebroside antibody produced in rabbit is suitable:
• for dot-blot immunoassay and western blot analysis of mouse brain homogenates.
• as a primary antibody at a working dilution of 1:50 for immunostaining of muscle tissue of Rana pipiens frogs
• as a primary antibody at a working dilution of 1:500 in immunofluorescence and immunohistochemistry using primary human astroglia
The antibody is useful for labeling myelin sheaths, oligodendrocytes and Schwann cells and for staining cultured cells, cell suspensions, frozen tissue sections and perfusion-fixed tissues. It is suitable for dot blot immunoassay at a working dilution of 1:50 using galactocerebroside type I and II.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence-cell culture cells (1 paper)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Galactocerebroside is the major galactosphingolipid of myelin. It is expressed in the CNS in cell membranes of oligodendrocytes. It is also expressed in Schwann cells in peripheral nerves. Galactocerebroside is found in serum and cerebral spinal fluid of patients with demyelinating diseases like multiple sclerosis.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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