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G9277 Sigma-Aldrich

Guanidine thiocyanate

for molecular biology, ≥99%

Synonym: Guanidinium rhodanide, Guanidinium thiocyanate

  • CAS Number 593-84-0

  • Linear Formula NH2C(=NH)NH2 · HSCN

  • Molecular Weight 118.16

  •  Beilstein/REAXYS Number 3563461

  •  EC Number 209-812-1

  •  MDL number MFCD00013027

  •  PubChem Substance ID 24895367

  •  NACRES NA.31



Related Categories Biochemicals, Biochemicals and Reagents, Chaotropic Agents, Core Bioreagents, DNA & RNA Purification,
Quality Level   200
biological source   synthetic (organic)
grade   for molecular biology
assay   ≥99%
form   powder or crystals
application(s)   RNA extraction: suitable
color   colorless to white,

mp   120-122 °C (lit.)
solubility   water: 100 mg/mL, clear, colorless
cation traces   Ba: ≤5 ppm
  Fe: ≤5 ppm
  Na: ≤0.5%
  heavy metals (as Pb): ≤5 ppm
Featured Industry   Agriculture
foreign activity   DNase, RNase, none detected
storage temp.   room temp
SMILES string   SC#N.NC(N)=N
InChI   1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H



Guanidine thiocyanate has been used:
• for the lysis of colorectal adenocarcinoma cell line prior to RNA isolation
• as a PCR inhibitor in triplex mitochondrial DNA (mtDNA) qPCR assay
• for DNA extraction from cerebrospinal fluid (CSF)

Chaotropic agent and strong denaturant; solubilizes cells.


100, 250, 500 g in poly bottle

Biochem/physiol Actions

Guanidine thiocyanate is a denaturing agent and is used routinely in RNA isolation. It is used as a storage buffer for whole blood samples. Guanidine thiocyanate inactivates nucleases and is ideal for storing and freezing fecal samples for DNA studies. It is used in combination with phenol−chloroform in RNA extraction.

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Supplemental Hazard Statements 
Personal Protective Equipment 
UN 1759 8 / PGIII
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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