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G9419 Sigma-Aldrich

Anti-GABAA Receptor (β1 subunit), Cytosolic Loop antibody produced in rabbit

~0.2 mg/mL, affinity isolated antibody, buffered aqueous glycerol solution

Synonym: Anti-γ-Aminobutyric Acid Type A Receptor (β1 subunit)

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to GABA and Glycine Receptors, Antibodies to Ion Channels,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 1:1,000 using rat membrane fraction
species reactivity   rat
mol wt   antigen apparent mol wt 51-54 kDa
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
usage   sufficient for 10 blots
concentration   ~0.2 mg/mL
UniProt accession no.   P15431
Gene Information   rat ... Gabrb1(25450)

Description

General description

GABAA and GABAB receptors differ with regard to their ionic characteristics and pharmacological properties. The GABAA receptor is an ionotropic receptor that forms the GABA gated chloride channel and consists of several heterogeneous subunits with membrane recognition sites for benzodiazapenes. GABAA receptor is a ligand-gated chloride channel that is a multimeric subunit complex. So far, around 16 subunits have been identified. This gene encodes the β1 subunit, which maps to human chromosome 4p12. It is present in a cluster along with genes encoding α 4, α 2 and γ 1 subunits of the GABA A receptor.

Immunogen

fusion protein with the amino acid sequence representing the cytosolic loop of the rat GABAA receptor (β1 subunit)

Application

Anti-GABAA Receptor (β1 subunit), Cytosolic Loop antibody produced in rabbit is suitable for western blotting at a working dilution of 1:1000 using rat membrane fraction.

Physical form

Solution in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

The inhibitory neurotransmitter GABA signals through two distinct types of pre- and postsynaptic receptors, GABAA and GABAB. Both GABA receptors can regulate depression of synaptic transmission and be involved in the inhibition controlling neuronal excitability. Disruption in this receptor is implicated in autism. Alterations in N-glycosylation of α1, β1, and β2 have been observed in schizophrenic patients.

Safety & Documentation

Safety Information

WGK Germany 
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Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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