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G9654 Sigma-Aldrich

Gentamicin-Glutamine solution

with 200 mM L-glutamine and 5 mg gentamicin per mL in tissue culture grade water, liquid, sterile-filtered, BioReagent, suitable for cell culture



Related Categories A - K, Aminoglycosides, Antibacterial, Antibiotic Solutions, Antibiotics,
sterility   sterile-filtered
product line   BioReagent
form   liquid
potency   5-6 mg per mL
application(s)   cell culture | mammalian: suitable
impurities   endotoxin, tested
suitability   suitable for cell culture
Mode of action   protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
shipped in   dry ice
storage temp.   −20°C



This solution is formulated to contain 200 mM L-glutamine and 5 mg/mL gentamicin in .9% sodium chloride


Gentamicn-Glutamine solution is used for selection of transformed cells that contain the aacA.aphD gene, and is recommended for use in cell culture applications at 10 mL/L.

Biochem/physiol Actions

Mode of Action: The product inhibits protein synthesis by binding to L6 protein of the 50S ribosomal subunit. Glutamine functions as an essential as an energy source for most mammalian cells in culture because of its amino acid carbon skeletons.

Antimicrobial spectrum: This product acts against both gram-negative and gram-positive bacteria, and mycoplasma.


This product is stable in solution at 37°C for 5 days. The recommended storage temperature is -20°C.

General description

Chemical structure: aminoglycoside

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

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