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  • G9672 - Anti-Par6γ (N-terminal) antibody produced in rabbit

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G9672 Sigma-Aldrich

Anti-Par6γ (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-PAR-6 partitioning defective 6 homolog gamma, Anti-PAR6G, Anti-PARD6G

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cellular Antigens,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 0.5-1 μg/mL using lysates of HEK-293T cells over-expressing Par6 gamma.
species reactivity   human
mol wt   antigen ~40 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   Q9BYG4
Gene Information   human ... PARD6G(84552)
mouse ... Pard6g(93737)
rat ... Pard6g(307237)

Description

General description

PARD6G (Par-6 family cell polarity regulator γ) is a cell polarity protein exists in three homolgues: α, β, and γ form. It is composed of a PDZ domain and a CRIB-like motif.

Immunogen

synthetic peptide corresponding to amino acids 2-16 of human Par6γ conjugated to KLH. The corresponding sequence is identical in mouse and rat.

Application

Anti-Par6γ (N-terminal) antibody produced in rabbit is suitable for western blot analysis at a working dilution of 0.5-1μg/mL using lysates of HEK-293T cells over-expressing Par6γ.

Target description

Anti-Par6γ (N-terminal) recognizes human Par6γ (alsoknown as PAR6D). Mammalian genomes contain three Par6 homologues (designated in humans Par6 α, β and γ). All three Par6 proteins are structurally similar, but seem to have different tissue expression patterns and subcellular distribution.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

The polarity complex, that is responsible for the establishment and maintenance of cell polarity, is composed of Par6, Par3 and atypical PKC isoforms (aPKC). These proteins have a key role in different cell polarization events that occur in vast range of species from worms to mammals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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