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GAGO20 Supelco

Glucose (GO) Assay Kit

sufficient for 20 assays

  •  NACRES NA.84

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Description

Application

This kit is for the quantitative, enzymatic determination of glucose in food and other materials. Glucose is oxidized to gluconic acid and hydrogen peroxide by glucose oxidase. Hydrogen peroxide reacts with o-dianisidine in the presence of peroxidase to form a colored product. Oxidized o-dianisidine reacts with sulfuric acid to form a more stable colored product. The intensity of the pink color measured at 540 nm is proportional to the original glucose concentration.

Suitability

Suitable for the quantitative, enzymatic determination of glucosein food and other materials.

Kit component only

Description

Product #

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o-dianisidine reagent 1 mL/vial    
glucose oxidase/peroxidase reagent 1 capsule    
glucose standard .5 mL    
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Can Product GAGO20, Glucose (GO) Assay Kit, be tested on blood or plasma glucose?
For analysis of such samples, this kit should be useful on samples that have been deproteinized.
Can Product GAGO20, Glucose (GO) Assay Kit, be used in a 96-well plate format?
Sigma Aldrich has not performed this in a 96-well plate format. However if a method has have not tried this. If you have developed a method in a 96-well format, Sigma Aldrich would be happy to know about this.
Can frozen samples be tested with Product GAGO20, Glucose (GO) Assay Kit?
One publication has successfully reported use of the GAGO20 on samples frozen at -20 °C.
CanProduct GAGO20, Glucose (GO) Assay Kit, be used on culture media samples?
Sigma Aldrich has not tested this product on culture media samples, but several publications in the literature report successful use of the kit with culture media samples.
What is the difference between the GAGO20 and GAHK20Glucose (GO) Assay Kits?
The kits use different enzymes. GAGO20 uses glucose oxidase and peroxidase, while GAHK20 uses hexokinase. Furthermore, the final reaction products are monitored at different wavelengths, 540 nm (GAGO20) and 340 nm (GAHK20).
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How wouldI decolorize my sample for use in the glucose assay using Product GAGO20, Glucose (GO)Assay Kit?
First, you will need to determine if the color affects the result by reading it as a blank in the assay.The classical method for decolorization of carbohydrate solutions is treatment with charcoal.A small amount of powdered charcoal such as ProductC3345 is added to the sample, stirred a few moments, then clarified by filtration or centrifugation. If the color persists, another portion of charcoal is added and the process repeated until there is no more change in color with an addition of charcoal.
My question is not addressed here, how can I contact Technical Service for assistance?
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Protocols & Articles

Articles

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Keywords: Absorption, Clinical, Degradations, Diabetes, Enzyme activity, Food Analysis, Nutrition, Obesity, Sample preparations

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Nuclear magnetic resonance (NMR) spectroscopy measures the extent to which a glycan or other molecule distorts a magnetic field. This technique is non-destructive and can provide full structural info...
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Keywords: Cancer, Digestions, Electrophoresis, Nuclear magnetic resonance spectroscopy, Separation, Spectroscopy

Peer-Reviewed Papers
15

References

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