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GAHK20 Supelco

Glucose (HK) Assay Kit

sufficient for 20 assays

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Description

Application

This kit is for the quantitative, enzymatic determination of glucose in food and other material. Glucose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phospho-gluconate in the presence of oxidized nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to glucose concentration.

Standard component

Description

Product #

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glucose (HK) assay reagent 20 mL    
glucose standard .5 mL    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Frequently Asked Questions

How do I determine what sample size to use in the Glucose (HK) Assay Kit, Product GAHK20?
If you know approximately how much glucose is present, dilute the sample down to 0.5 to 50 ug of glucose and use between 10 to 200 ul sample volume. If you do not know how much glucose is present, you should assay the undiluted sample and dilutions of it. Based on the dilution factor you can determine the glucose concentration.
Can the procedure for Glucose (HK) Assay Kit, Product GAHK20,be scaled down?
Although we have not done it, you should be able to scale down the procedure as long as you have pipettes capable of dispensing accurate ul volume quantities and an instrument that can monitor the 340 nm wavelength.
If my sample for the Glucose (HK) Assay Kit, Product GAHK20,has a lot of protein, how do I deproteinize it?
You can deproteinize by using barium hydroxide, B4059, and zinc sulfate, Z2876, by adding 0.2 ml of sample to 1.8 ml of water, swirl and mix, then 1.0 ml of barium hydroxide, swirl and mix, then 1.0 ml of zinc sulfate, swirl and mix thoroughly by shaking, and then filter or centrifuge to obtain clear filtrate.
How do I decolorize a solution for use with Product GAHK20, Glucose (HK) Assay Kit?
You can decolorize a solution by adding a small amount of charcoal to the solution, swirl, then filter or centrifuge to remove charcoal.
How do I degas a solution for use with Product GAHK20, Glucose (HK) Assay Kit?
You can degas a solution by either putting it under a vacuum or by bubbling nitrogen through it.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
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Peer-Reviewed Papers
15

References

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