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GE10600121

Amersham Hybond® P Western blotting membrane sandwich, PVDF

pore size 0.45 μm, sheet W × L 80 mm × 90 mm , preassembled with 2 x 3MM chr filter papers, pkg of 10 ea

  •  NACRES NB.22

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Properties

Related Categories Hybond PVDF membranes, Labware, Manufacturer Browser, Whatman, Whatman Blotting / Electrophoresis Supplies,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pkg of 10 ea
mfr. no.   Cytiva, 10600121
sheet W × L   80 mm × 90 mm , preassembled with 2 x 3MM chr filter papers
pore size   0.45 μm pore size

Description

General description

PVDF membranes have higher mechanical strength than NC membranes and, thus, are more suitable for Western blotting applications where stripping and reprobing is needed. In addition, PVDF membranes exhibit higher protein binding capacity, however, with a tendency for a higher background, compared with NC membranes.

Legal Information

Amersham is a trademark of Cytiva

Hybond is a registered trademark of Cytiva

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Detection of GST-tagged Proteins with Western Blot

Expression and purification of GST-tagged proteins can be monitored by Western blot analysis, using Amersham ECL, Amersham ECL Prime, or Amersham ECL Select detection systems to enhance sensitivity.
Keywords: Electrophoresis, Gene expression, PAGE, Purification, Separation, Tagged proteins, Western blot

Troubleshooting of Detection Methods of GST-tagged Proteins

The troubleshooting guide below addresses problems common to the majority of detection methods as well as problems specific to a particular method. In the latter case, the relevant method is indicate...
Keywords: Buffers, Cell disruption, Detection methods, Electroblotting, Gene expression, Purification, Tagged proteins, Western blot

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