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GE11-0034-00

His Buffer Kit

Cytiva, 11-0034-00

  •  NACRES NA.25

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Properties

shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pkg of 1 ea
mfr. no.   Cytiva, 11-0034-00

Description

General description

His Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of histidine-tagged proteins according to recommended protocols. Moreover, method for optimization of imidazole concentration in the binding buffer is described.

Features and Benefits

His Buffer Kit can be used in combination with a number of column types, such as His SpinTrap, His MultiTrap FF, His MultiTrap HP, His GraviTrap, HisTrap FF, HisTrap FF crude and HisTrap HP

Storage and Stability

Store at 4 to 30 °C ()

Analysis Note

www.gelifesciences.com.

Legal Information

HisTrap is a trademark of Cytiva

MultiTrap is a trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Protocols

Characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and Uncharged IMAC Sepharose Products

Appendix 1, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016  
Keywords: Affinity chromatography, Buffers, Cell culture, Centrifugation, Chromatography, Detergents, Immobilization, Precipitation, Purification, Tagged proteins

Gravity-flow purification using His GraviTrap and His GraviTrap Kit

His GraviTrap™ columns are designed for fast and simple purification of histidine-tagged proteins using gravity flow. Both clarified and unclarified sample can be applied to the column. The column is pre...
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, PAGE, Precipitation, Sequencing, Sonication, Tagged proteins, Western blot

Magnetic Bead-Based Purification/Screening Using His Mag Sepharose Ni

His Mag Sepharose® Ni is a magnetic-bead-based IMAC medium charged with nickel ions. It is designed for efficient, small-scale purification/screening of histidine-tagged proteins from various sources. ...
Keywords: Affinity chromatography, Buffers, Cell culture, Chromatography, Immobilization, Separation, Tagged proteins

Magnetic Bead-Based Purification of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using His Mag Sepharose Excel

His Mag Sepharose® excel is a magnetic-bead-based IMAC medium charged with nickel ions. The magnetic beads are designed for simple, small-scale capture and purification of histidine-tagged proteins se...
Keywords: Affinity chromatography, Cell culture, Chromatography, Degradations, Ligands, Separation, Tagged proteins

Minipreps using His SpinTrap and His SpinTrap Kit

His SpinTrap™ and His SpinTrap™ Kit are designed for efficient minipreps (small-scale purification) of histidine-tagged proteins directly from clarified or unclarified cell lysates. The columns may also ...
Keywords: Affinity chromatography, Buffers, Cell culture, Cell disruption, Centrifugation, Chromatography, Degradations, Gene expression, Homogenization, PAGE, Sample preparations, Sonication, Tagged proteins

Optimizing Purification of Histidine-Tagged Proteins

Chapter 4, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Immobilization, Ion Exchange, PAGE, Purification, Separation, Sequencing, Size-exclusion chromatography, Tagged proteins

Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose High Performance

Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions. The chromatography medi...
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Filtration, Homogenization, Immobilization, Purification, Sample preparations, Sonication, Tagged proteins

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