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Sephadex® G-25 Medium

GE Healthcare, 17-0033-02, pack of 500 g

  •  NACRES NA.56



Related Categories Analytical/Chromatography, GE Healthcare Life Sciences Columns and Media, GE Life Sciences Purification Media, Gel Filtration Chromatography, Gel Filtration Media,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 500 g
mfr. no.   GE Healthcare, 17-0033-02
storage condition   dry media
matrix   cross-linked dextran
particle size   >50 μm (dry)
  85-260 μm
cleaning in place   2 - 13
working range   2 - 13
suitability   suitable for bioprocess medium


General description

Sephadex® G-25 is well established gel filtration medium for desalting and buffer exchange in industrial applications.

Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-25 is available in 4 different particle sizes (Course, Medium, Fine & Superfine).

Sephadex® G-25 is available in different bulk pack sizes and convenient pre-packed formats.
As a BioProcess medium, Sephadex® G-25, meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

• Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
• Excellent recovery and minimum sample dilution
• Available in prepacked HiPrep Desalting and HiTrap® Desalting columns for fast and convenient desalting
• BioProcess medium supported for industrial applications and well-established for desalting and buffer exchange in industrial applications

Storage and Stability

Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

HiPrep is a trademark of Cytiva

HiTrap is a registered trademark of Cytiva

Sephadex is a registered trademark of Cytiva

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

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Protocols & Articles


Affinity Chromatography Troubleshooting

This section focuses on practical problems that may occur when running a chromatography column. The diagrams below give an indication of how a chromatogram may deviate from the ideal during affnity p...
Keywords: Adsorption, Affinity chromatography, Buffers, Chromatography, Detergents, Digestions, Fractionation, Ligands, PAGE, Precipitation, Purification, Sample preparations, Separation, Solvents

Buffer Exchange and Desalting for Affinity Chromatography

Dialysis is frequently mentioned in the literature as a technique to remove salt or other small molecules and to exchange the buffer composition of a sample. However, dialysis is generally a very slo...
Keywords: Absorption, Affinity chromatography, Buffers, Chromatography, Dialysis, Separation

Fractional Precipitation of Affinity Chromatography Samples

Appendix 1 extracted from Affinity Chromatography Principles and Methods GE Healthcare, 2007
Keywords: Affinity chromatography, Centrifugation, Chromatography, Electrophoresis, Nucleic acid denaturation, PAGE, Precipitation, Purification

Sample Preparation for Affinity Chromatography

Appendix 1 extracted from Affinity Chromatography Principles and Methods, GE Healthcare, 2007
Keywords: Adsorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Filtration, Nucleic acid denaturation, PAGE, Precipitation, Sample preparations

Sephadex and Darcy’s Law

Appendix 2, Extracted from Size Exclusion Chromatography Principles and Methods (PDF) GE Healthcare, 2014
Keywords: Chromatography, Size-exclusion chromatography

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Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

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