• USA Home
  • GE17-0042-01 - Sephadex® G-50

EMAIL THIS PAGE TO A FRIEND
GE17-0042-01

Sephadex® G-50

Fine, GE Healthcare, pack of 100 g

Purchase

Properties

Related Categories Analytical/Chromatography, GE Healthcare Life Sciences Columns and Media, GE Life Sciences Purification Media, Gel Filtration Chromatography, Gel Filtration Media,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
feature   autoclavable In wet form (pH 7.0) at 120°C for 30 min
packaging   pack of 100 g
mfr. no.   GE Healthcare
parameter   flow rate
matrix   cross-linked dextran
swelling   1 g swells to 9-11 mL
particle size   20-80 μm (dry)
  (wet)
cleaning in place   2 - 13
working range   2 - 10

Description

General description

Sephadex® G-50 Fine is well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The Fine´s small bead size give higher efficiency.

Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations at where high flow rates and low operating pressures are required.

Features and Benefits

• Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
• Suitable for purification of DNA from small molecules by gel filtration
• Small bead size for shorter diffusion distances
• Classic gel filtration medium.

Storage and Stability

Store at 4 to 30 °C ()

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

Sephadex is a registered trademark of GE Healthcare

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Protocols

Sephadex and Darcy’s Law

Appendix 2, Extracted from Size Exclusion Chromatography Principles and Methods (PDF) GE Healthcare, 2014
Keywords: Chromatography, Size-exclusion chromatography

Related Content

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?