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Sephadex® G-50

Fine, GE Healthcare, pack of 100 g



Related Categories Analytical/Chromatography, GE Healthcare Life Sciences Columns and Media, GE Life Sciences Purification Media, Gel Filtration Chromatography, Gel Filtration Media,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
feature   autoclavable In wet form (pH 7.0) at 120°C for 30 min
packaging   pack of 100 g
mfr. no.   GE Healthcare
parameter   flow rate
matrix   cross-linked dextran
swelling   1 g swells to 9-11 mL
particle size   20-80 μm (dry)
cleaning in place   2 - 13
working range   2 - 10


General description

Sephadex® G-50 Fine is well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The Fine´s small bead size give higher efficiency.

Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations at where high flow rates and low operating pressures are required.

Features and Benefits

• Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
• Suitable for purification of DNA from small molecules by gel filtration
• Small bead size for shorter diffusion distances
• Classic gel filtration medium.

Storage and Stability

Store at 4 to 30 °C ()

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

Sephadex is a registered trademark of Cytiva

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles


Sephadex and Darcy’s Law

Appendix 2, Extracted from Size Exclusion Chromatography Principles and Methods (PDF) GE Healthcare, 2014
Keywords: Chromatography, Size-exclusion chromatography

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