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GE17-0050-01

Sephadex® G-75

GE Healthcare, 17-0050-01, pack of 100 g

  •  NACRES NA.56

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Properties

Related Categories Analytical/Chromatography, GE Healthcare Life Sciences Columns and Media, GE Life Sciences Purification Media, Gel Filtration Chromatography, Gel Filtration Media,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
feature   autoclavable In wet form (pH 7.0) at 120°C for 30 min
packaging   pack of 100 g
mfr. no.   GE Healthcare, 17-0050-01
parameter   flow rate (The beads behave as rigid spheres obeying Darcy′s Law.)
  flow rate
matrix   cross-linked dextran
particle size   40-120 μm (dry)
  90-280 μm (wet)
working range   2 - 10

Description

General description

Sephadex® G-75 is well established gel filtration medium for desalting and buffer exchange of large biomolecules >80 000 molecular weight.

Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-10 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.

Features and Benefits

• Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
• Suitable for buffer exchange and cleanup of large biomolecules, >80 000 molecular weight
• Highest exclusion limit, 80 000, in the Sephadex® G-range
• Classic gel filtration medium.

Storage and Stability

Store at 4 to 30 °C ()

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

Sephadex is a registered trademark of GE Healthcare

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Performing a Separation with Sephadex

Extracted from Size Exclusion Chromatography Principles and Methods, (PDF) GE Healthcare, 2014
Keywords: Buffers, Centrifugation, Chromatography, Detergents, Fractionation, PAGE, Precipitation, Purification, Sample preparations, Separation, Size-exclusion chromatography

Separation options with Sephadex

Extracted from Size Exclusion Chromatography Principles and Methods, (PDF) GE Healthcare, 2014
Keywords: Chromatography, Separation

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