• USA Home
  • GE17-0050-01 - Sephadex® G-75


Sephadex® G-75

GE Healthcare, 17-0050-01, pack of 100 g

  •  NACRES NA.56



Related Categories Analytical/Chromatography, GE Healthcare Life Sciences Columns and Media, GE Life Sciences Purification Media, Gel Filtration Chromatography, Gel Filtration Media,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
feature   autoclavable In wet form (pH 7.0) at 120°C for 30 min
packaging   pack of 100 g
mfr. no.   GE Healthcare, 17-0050-01
parameter   flow rate (The beads behave as rigid spheres obeying Darcy′s Law.)
  flow rate
matrix   cross-linked dextran
particle size   40-120 μm (dry)
  90-280 μm (wet)
working range   2 - 10


General description

Sephadex® G-75 is well established gel filtration medium for desalting and buffer exchange of large biomolecules >80 000 molecular weight.

Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-10 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.

Features and Benefits

• Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
• Suitable for buffer exchange and cleanup of large biomolecules, >80 000 molecular weight
• Highest exclusion limit, 80 000, in the Sephadex® G-range
• Classic gel filtration medium.

Storage and Stability

Store at 4 to 30 °C ()

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

Sephadex is a registered trademark of GE Healthcare

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles


Performing a Separation with Sephadex

Extracted from Size Exclusion Chromatography Principles and Methods, (PDF) GE Healthcare, 2014
Keywords: Buffers, Centrifugation, Chromatography, Detergents, Fractionation, PAGE, Precipitation, Purification, Sample preparations, Separation, Size-exclusion chromatography

Separation options with Sephadex

Extracted from Size Exclusion Chromatography Principles and Methods, (PDF) GE Healthcare, 2014
Keywords: Chromatography, Separation

Related Content

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?