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GE17-0090-01

Sephadex® LH-20

GE Healthcare, 17-0090-01, pack of 100 g

  •  NACRES NA.56

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Properties

Related Categories Analytical/Chromatography, GE Healthcare Life Sciences Columns and Media, GE Life Sciences Purification Media, Gel Filtration Chromatography, Gel Filtration Media,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
feature   autoclavable 20 min at 121°C
packaging   pack of 100 g
mfr. no.   GE Healthcare, 17-0090-01
matrix   hydroxypropylated, cross-linked dextran
particle size   163 μm (in methanol max.)
  18-111 μm (dry)
  27 μm (In Methanol Min.)
cleaning in place   2 - 13
working range   2 - 13

Description

General description

Sephadex® LH20 is a liquid chromatography media for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. Useful to analytical and industrial scale for preparation of closely related molecular species.

Sephadex® LH-20 is prepared by hydroxypropylation of Sephadex® G-25, a bead-formed dextran medium, and has been specifically developed for gel filtration of natural products, such as steroids, terpenoids, lipids and low molecular weight peptides, in organic solvents.

Sephadex® LH-20 is useful at both analytical and industrial scale for the preparation of closely related molecular species. Due to the physico-chemical properties of this media, it can be used either during initial purification prior to polishing by high performance ion exchange or reversed phase chromatography or as the final polishing step e.g. during the preparation of diastereomers. Sephadex® LH-20 is characterized by (1) Chromatographic selectivity due to dual hydrophilic and lipophilic nature of the matrix (2) Easily predicted elution behaviour based on the chemical structure 1–3, 16 of the sample (3) Chemical and physical robustness (4) Excellent batch to batch reproducibility

Features and Benefits

• Designed for use in polar organic solvents and aqueous solvent mixtures
• Excellent for fractionation of lipids, hormones, vitamins, and other small biomolecules.
• Useful for both analytical and industrial scales for the preparation of closely related molecular species
• Sephadex® LH-20 is made from hydroxypropylated dextran beads that have been crosslinked to yield a polysaccharide network.
• Powerful chromatographic selectivity due to dual hydrophilic and lipophilic nature of the matrix
• Excellent batch-to-batch reproducibility
• Sephadex® LH-20 is made from hydroxypropylated dextran beads that have been crosslinked to yield a polysaccharide network.

Storage and Stability

Store at 4 to 25 °C ()

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

Sephadex is a registered trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Packing a column and Performing a Separation with Sephadex LH-20

Extracted from Size Exclusion Chromatography Principles and Methods, (PDF) GE Healthcare, 2014
Keywords: Chromatography, Separation, Solvents

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Peer-Reviewed Papers
15

References

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