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Sepharose® 6B

Cytiva, 17-0110-01, pack of 1 L

Synonym: Agarose, Beaded



Related Categories Analytical/Chromatography, Cytiva Columns and Media, Cytiva Purification Media, HPLC & FPLC Columns for Biopolymer Separations, HPLC/UHPLC,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
feature   autoclavable Autoclavable, 20 min at 120°C in pH 7
packaging   pack of 1 L
mfr. no.   Cytiva, 17-0110-01
matrix   6% agarose
particle size   45-165 μm
cleaning in place   4 - 9
working range   4 - 9
SMILES string   OC[C@H]1O[C@@H](O[C@@H]2[C@@H]3CO[C@H]2[C@H](O)[C@@H](O3)O[C@H]4[C@@H](O)[C@@H](CO)O[C@@H](O[C@@H]5[C@@H]6CO[C@H]5[C@H](O)[C@H](O)O6)[C@@H]4O)[C@H](O)[C@@H](O)[C@H]1O
InChI   1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1


General description

Sepharose® 6B is a well-proven agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

Sepharose® is a bead-formed agarose-based gel filtration matrix. Sepharose® is available with 3 different agarose contents; 2, 4, and 6%, designated Sepharose® 2B, Sepharose® 4B and Sepharose® 6B respectively, Both Sepharose® and Sepharose® CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight.

Sepharose® CL gels are cross-linked derivatives of Sepharose® 2B, Sepharose® 4B and Sepharose® 6B. The cross-linked form of Sepharose® is chemically and physically more resistant than Sepharose® itself, offering the same selectivity with better flow characteristics. Cross-linked Sepharose® gels are resistant to organic solvents and are thus the choice for separations in organic solvents.

Features and Benefits

• 6% Agarose gel filtration media.
• Proven base matrix for coupling affinity ligands

Other Notes

Beaded agarose for fractionating molecules of high molecular weight. Cross-linked beaded agarose is more resistant to denaturing conditions, and thus offers more versatility in the choice of sample buffer and eluent. The approximate % agarose concentration is indicated by the product name (Ultrogel A%).

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

Sepharose is a registered trademark of Cytiva

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

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Protocols & Articles

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