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GE17-0300-10

Ficoll® PM 400

Cytiva, 17-0300-10, pack of 100 g

  •  NACRES NA.25

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Properties

Related Categories Cell Fractionation and Organelle Isolation, Centrifugation, Molecular Biology, Native Protein Sample Preparation, Proteomics More...
form   solid
  spray-dried
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 100 g
mfr. no.   Cytiva, 17-0300-10
color   White
solubility   soluble (It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be attained)

Description

General description

High molecular weight sucrose-polymers formed by copolymerization of sucrose with epichlorohydrin. The molecules are highly branched and the high content of hydroxyl groups leads to very good solubility in aqueous media.

Features and Benefits

• For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.
• Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution.
• Concentrations of up to 50% (w/v) covering densities of up to 1.2 g/mL can be achieved.
• Better osmotic properties than sucrose.
• Preserves functional and morphological integrity.
• Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size (under conditions of sedimentation at unit gravity).
• Does not penetrate biological membranes.
• Serves as the raw material for preparation of Ficoll-Paque gradients.
• Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier.

Storage and Stability

Store at 4 to 30 °C ()

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Ficoll is a registered trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

A Recommended Standard Method for Isolating Mononuclear Cells

Cell separation using Ficoll-Paque products can be carried out over a wide range of blood sample volumes. With its high yield, this method can be adapted to the processing of very small amounts of bl...
Keywords: Anticoagulants, Cancer, Cell culture, Cell disruption, Cell proliferation, Centrifugation, Culture media, Gene expression, PAGE, Purification, Separation, Sterilizations

Applications of Percoll In Blood Cells

The entire spectrum of cell types present in blood can be resolved on preformed gradients of Percoll. The method described by Pertoft et al. (55) (Fig 19) utilizes both rate zonal (separation by size...
Keywords: AGE, Adhesion, Apoptosis, Cell culture, Cell proliferation, Centrifugation, Coagulation, Dialysis, Digestions, Enzyme activity, Filtration, Flow cytometry, Fractionation, Immunoassay, Lipid peroxidation, Metabolism, Microscopy, Northern blot, Nucleic acid hybridization, Peroxidations, Phosphorylations, Polymerase chain reaction, Purification, Scanning electron microscopy, Separation, Vitamins, Western blot

Applications of Percoll In Other Cell Types

The following tables were compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll for a particular cell or tissue type.
Keywords: Apoptosis, Cell culture, Cell purification, Centrifugation, Diffusion, Electrophoresis, Flow cytometry, Fractionation, Gel electrophoresis, Growth factors, Hormones, Immunocytochemistry, Immunofluorescence, In Situ hybridization, Metabolism, Microscopy, Nucleic acid hybridization, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Protein assay, Purification, Scanning electron microscopy, Separation, Southern blot

How to fractionate and analyze gradients of Percoll

Density Marker Beads are dyed derivatives of Sephadex™. There are ten color-coded bead types, each with a specific density. They have been specifically formulated for use in Percoll gradients and wil...
Keywords: Centrifugation, Fractionation, PAGE, Precipitation

How to make and use gradients of Percoll

In order to use Percoll to prepare a gradient, the osmolality of Percoll (undiluted) must first be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutio...
Keywords: Addition reactions, Buffers, Cell culture, Centrifugation, Culture media, Fractionation, PAGE, Purification, Separation

Properties of mononuclear cells isolated by the Ficoll-Paque method

Since its introduction in 1968, the mononuclear cell separation method described by Bøyum 1, 2 has been used in numerous immunological investigations. This widespread adoption indicates the superior ...
Keywords: Adsorption, Gene expression, Separation

Removal of Percoll after centrifugation

Since Percoll is non-toxic to biological materials and does not adhere to membranes, it is usually unnecessary to remove Percoll from the purified preparation. Cells can be transferred directly to ce...
Keywords: Cell culture, Centrifugation, Chromatography, Electrophoresis, Filtration, Microscopy, Scanning electron microscopy

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