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GE17-0440-01 Sigma-Aldrich

Con-A Sepharose® 4B

GE Healthcare, 17-0440-01, pack of 100 mL

  •  NACRES NA.56

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Properties

Related Categories Activated/Functionalized Matrices, Affinity Chromatography, Carbohydrate Binding Matrices, Molecular Biology, Protein Chromatography,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 100 mL
mfr. no.   GE Healthcare, 17-0440-01
matrix   4% agarose
particle size   45-165 μm
average diameter   90 μm
cleaning in place   4 - 9
working range   4 - 6
storage temp.   2-8°C

Description

General description

Con A Sepharose® 4B with immobilized Concanavalin A is an affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids. Stronger affinity for α-D-glucose than α-D-mannose.

Con A Sepharose® is Concanavalin A coupled to Sepharose® 4B by the cyanogen bromide method. Concanavalin A (Con A) is a tetrameric metalloprotein isolated from Canavalia ensiformis (jack bean). Con A binds molcules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose® is routinely used for separation and purification of glycoproteins, polysaccharides and glycolipids.

Features and Benefits

• Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
• Group-specific adsorbent for molecules containing sugars.
• Highly suitable for isolation of cell surface glycoproteins from detergent-solubilized membranes
• Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
• Highly suitable for isolation of cell surface glycoproteins from detergent-solubilized membranes
• Con A coupled to Sepharose® 4B via cyanogen bromide activation

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

Sepharose is a registered trademark of GE Healthcare

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Converting from Flow Velocity to Volumetric Flow Rates

Appendix 7, Extracted from Affinity Chromatography Vol. 1: Antibodies, GE Healthcare, 2016  
Keywords: Affinity chromatography, Chromatography

Protocols

Affinity Chromatography Troubleshooting

This section focuses on practical problems that may occur when running a chromatography column. The diagrams below give an indication of how a chromatogram may deviate from the ideal during affnity p...
Keywords: Adsorption, Affinity chromatography, Buffers, Chromatography, Detergents, Digestions, Fractionation, Ligands, PAGE, Precipitation, Purification, Sample preparations, Separation, Solvents

Column Packing and Preparation for Affinity Chromatography with Specific Groups of Biomolecules

Appendix 3, extracted from Affinity Chromatography Vol. 3: Specific Groups of Biomolecules, GE Healthcare, 2014
Keywords: Antimicrobials, Chromatography, Gene expression, Separation

Column Packing and Preparation for Affinity Chromatography

Appendix 3,  Extracted from Affinity Chromatography Principles and Methods, GE Healthcare, 2007
Keywords: Affinity chromatography, Antimicrobials, Buffers, Chromatography, Separation

Performing a Purification of Branched Mannoses, Carbohydrates with Terminal Mannose or Glucose (αMan > αGlc > GlcNAc) with Con A Sepharose 4B

Concanavalin A (Con A) is a tetrameric metalloprotein isolated from Canavalia ensiformis (jack bean). Con A binds molecules containing a-D-mannopyranosyl, a-D-glucopyranosyl and sterically related re...
Keywords: Absorption, Affinity chromatography, Buffers, Chromatography, Detergents, Ligands, Separation

Purification or Removal of Glycoproteins and Polysaccharides

Extracted from Affinity Chromatography Vol. 3: Specific Groups of Biomolecules, GE Healthcare, 2014
Keywords: Absorption, Buffers, Chromatography, Detergents, Ligands, PAGE, Separation

Sample Preparation for Affinity Chromatography

Appendix 1 extracted from Affinity Chromatography Principles and Methods, GE Healthcare, 2007
Keywords: Adsorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Filtration, Nucleic acid denaturation, PAGE, Precipitation, Sample preparations

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