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CM Sepharose® Fast Flow

Cytiva, 17-0719-10, pack of 25 mL

  •  NACRES NA.56



description   Ion Exchanger Type (value)
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 25 mL
mfr. no.   Cytiva, 17-0719-10
matrix   6% cross-linked agarose
particle size   45-165 μm
average diameter   90 μm
cleaning in place   2 - 14
working range   4 - 13
suitability   suitable for bioprocess medium


General description

CM Sepharose® Fast FLow is a weak cation exchanger based on the well established Sepharose® Fast FLow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.


CM Sepharose® Fast FLow is part of the Sepharose® Fast FLow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of crosslinked 6% Agarose beads, with carboxymethyl (CM) weak cation exchange groups. CM Sepharose® Fast FLow has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with CM Sepharose® Fast FLow is straight forward and the medium is available in a range of pre-packed process development tools.

As member of the BioProcess media range, CM Sepharose® Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

• Well-proven weak cation exchanger developed for industrial downstream processes
• Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
• The industry standard for ion exchange chromatography during recent decades
• High chemical stability allows for well proven CIP and sanitization protocols
• The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a registered trademark of Cytiva

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles


Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

Correct preparation of samples and buffers and application of a high salt wash (1 M NaCl) at the end of each separation should keep most columns in good condition. However, reduced performance, a slo...
Keywords: Buffers, Detergents, Separation, Solvents

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

Prepacked columns from Cytiva will ensure reproducible results and the highest performance.
Keywords: Antimicrobials, Chromatography, Gene expression, Purification, Separation

Ion Exchange Chromatography Troubleshooting

If only certain peaks are of interest in this well-resolved separation, it may be advantageous to transfer to a step elution in order to save time and buffer. The rest of this section focuses on prac...
Keywords: Absorption, Adsorption, Buffers, Chromatography, Detergents, Digestions, PAGE, Precipitation, Sample preparations, Separation, Solvents

Non-volatile and Volatile Buffer Systems

From our library of Protocols, Sigma-Aldrich presents Non-volatile and Volatile Buffer Systems

Practical Considerations for IEX Separation

This section covers detailed aspects of each step in an IEX separation, together with practical hints and tips to improve resolution and overall performance. In practice a separation can be summarize...
Keywords: Absorption, Buffers, Cell culture, Chromatography, Detergents, Filtration, Ion Exchange, Nucleic acid denaturation, PAGE, Phase transitions, Precipitation, Sample preparations, Separation, Solvents

Sample Preparation in Ion Exchange Chromatography

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, may reduce th...
Keywords: Absorption, Adsorption, Affinity chromatography, Amplification, Buffers, Cell culture, Centrifugation, Chromatography, Detergents, Dialysis, Electrophoresis, Filtration, Indicators, Nucleic acid denaturation, PAGE, Precipitation, Purification, Sample preparations, Separation

Selection of Purification Equipment

Simple IEX chromatography, such as elution by a step-gradient, can be performed using a syringe or peristaltic pump with prepacked HiTrap columns. A chromatography system is required in order to achi...
Keywords: Chromatography, Purification, Separation

Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up

Use Sepharose Fast Flow for capture or intermediate purification steps that require good resolution (flows up to 300 cm/h).
Keywords: Buffers, Chromatography, Detergents, Filtration, High performance liquid chromatography, Ion Exchange, PAGE, Precipitation, Purification, Sample preparations, Separation, Solvents

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