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GE17-0727-20

SOURCE 15RPC

Cytiva, 17-0727-20, pack of 10 mL

  •  NACRES NA.56

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Properties

shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
feature   autoclavable 20 min at 121°C
packaging   pack of 10 mL
mfr. no.   Cytiva, 17-0727-20
matrix   polystyrene/divinylbenzene
avg. part. size   15 μm (monosized)
cleaning in place   1 - 14
working range   1 - 12
capacity   ~10 mg binding capacity(~10 mg BSA /ml medium at 300 cm/h, ~30 mg bacitracin/ml medium at 300 cm/h, ~50 mg insulin/ml medium at 300 cm/h)
suitability   suitable for bioprocess medium

Description

General description

SOURCE 15RPC is designed for reversed phase chromatography (RPC) with full pH range stability and suitable for polishing steps in industrial processes requiring high f low velocities and low back-pressure.

Application

SOURCE 15RPC is based on a 15 μm, monosized, rigid polystyrene/divinyl benzene matrix. This gives Low back pressures and high flow rates during operation allowing for high productivity. The 15 μm bead size allows for very high resolution chromatography steps.

High quality assurance standards in combination with the patented production method, particle structure is consistent both bead-to-bead and batch-to-batch.

A controlled pore size distribution and large specific surface area offer excellent capacity for a wide range of molecules, from small peptides and oligonucleotides up to large Proteins. With full pH range stability, SOURCE 15RPC is a strong alternative to silica-based RPC matrices.

SOURCE 15RPC is available in a range of different bulk pack sizes for easy scale-up and process development. As member of the BioProcess media range, SOURCE 15RPC meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

• Mono-sized 15 μm rigid beads gives Low back pressures and high flow rates allowing for high productivity.
• Designed for high resolution reversed phase chromatography of Proteins, peptides and oligonucleotides.
• High chemical stability allowing for wide range of working conditions with good resistance to cleaning conditions at high pH
• BioProcess medium supported for industrial applications.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol or 70% Acetonitrile)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

SOURCE is a trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Protocols

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

Prepacked columns from Cytiva will ensure reproducible results and the highest performance.
Keywords: Antimicrobials, Chromatography, Gene expression, Purification, Reversed-phase chromatography, Separation

HIC as a Capture Step

The objective of a capture step is to quickly bind the protein(s) of interest from the crude sample and isolate them from critical contaminants such as proteases and glycosidases. The target protein(...
Keywords: Cell culture, Filtration, Growth factors, Purification, Reversed-phase chromatography, Separation

Performing an RPC Separation with SOURCE™ Media

Check column performance regularly by determining column efficiency and peak symmetry. See Appendix 2.
Keywords: Buffers, Chromatography, Detergents, Filtration, Reversed-phase chromatography, Separation, Solvents

RPC Purification Options and Scale up with SOURCE™ Media

SOURCE™ media are based on a matrix made from monodispersed, rigid, polystyrene/divinyl benzene (Figure 80). The media demonstrate extreme chemical and physical stability and, unlike silica-based med...
Keywords: Alzheimer Disease, Chromatography, Growth factors, High performance liquid chromatography, Ion Exchange, Liquid chromatography mass spectrometry, Mass spectrometry, Polymerization reactions, Purification, Reversed-phase chromatography, Separation

Reverse Phased Chromatography (RPC) in Practice

As discussed under the theoretical section, a successful RPC separation is influenced by many parameters and will always need to be optimized to meet the requirements for the application. Steps towar...
Keywords: Chromatography, Evaporation, Fractionation, High performance liquid chromatography, Nucleic acid denaturation, PAGE, Phase transitions, Purification, Reversed-phase chromatography, Separation, Solvents, Sonication

Selection of Purifiation Equipment for Hydrophobic Interaction and Reversed Phase Chromatography

A chromatography system is required in order to achieve a high-resolution separation using accurately controlled linear gradient elution, to take advantage of the high flow rates of modern media, or ...
Keywords: Chromatography, Mass spectrometry, Separation

Troubleshooting Reversed Phase Chromatography (RPC)

Poor-quality eluent components can cause a phenomenon referred to as “ghosting”. Trace levels of organic impurities bind to the medium, concentrating during equilibration and sample application. When...
Keywords: Absorption, Chromatography, Evaporation, High performance liquid chromatography, Phase transitions, Precipitation, Reversed-phase chromatography, Sample preparations, Separation

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