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GE17-1043-01

Superdex® 200 Prep Grade

Cytiva, 17-1043-01, pack of 150 mL

  •  NACRES NA.56

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Properties

Related Categories Analytical/Chromatography, Cytiva Columns and Media, Cytiva Purification Media, HPLC & FPLC Columns for Biopolymer Separations, HPLC/UHPLC,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 150 mL
mfr. no.   Cytiva, 17-1043-01
matrix   composite of cross-linked agarose and dextran
particle size   24-44 μm
avg. part. size   34 μm
cleaning in place   2 - 14
working range   3 - 12
suitability   suitable for bioprocess medium

Description

General description

Superdex® 200 prep grade gel filtration medium offers very high resolution fractionation for separation, polishing and product formulation of monoclonal antibodies.

Superdex® prep grade is a preparative gel filtration medium with a composite matrix of dextran and agarose. This matrix combines the excellent gel filtration properties of cross-linked dextran (Sephadex) with the physical and chemical stabilities of highly cross-linked agarose, to produce a separation medium with outstanding selectivity and high resolution. In addition, its low non-specific interaction permits high recovery of biological material. Together these properties make Superdex® prep grade the first choice gel filtration media for all applications from laboratory to process scale.

Superdex® prep grade is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As a BioProcess medium, Superdex® prep grade meets industrial demands with security of supply and comprehensive technical and regulatory support.

Superdex® prep grade is available in three selectivities:
Superdex® 30 prep grade is designed for purification of molecules in the molecular weight range up to 10 000 MW, such as peptides, oligosaccharides and small proteins.
Superdex® 75 prep grade is recommended for the purification of recombinant proteins. (Fractionation range 3000-70000 Da).
Superdex® 200 prep grade is recommended for the purification of monoclonal antibodies. (Fractionation range 10 000 -600 000 Da)

Features and Benefits

• Gel filtration medium providing exceptional resolution with short run times and good recovery.
• Superdex® 200 prep grade is recommended for the purification of monoclonal antibodies.
• BioProcess medium supported for industrial applications and well-established in approved processes.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Superdex is a registered trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Two-step purification of mouse monoclonal IgG1 for diagnostic use

The goal of this example was purification of a monoclonal antibody to achieve a level of purity sufficient for in vitro diagnostic use. The two-step procedure combined hydrophobic interaction chromatog...
Keywords: Cell culture, Chromatography, Diagnostic, Size-exclusion chromatography

Protocols

Purification using MBPTrap HP Columns

MBPTrap HP 1 ml and 5 ml columns (Fig 6.2) are made of biocompatible polypropylene that does not interact with biomolecules. Prepacked MBPTrap HP columns provide fast, simple, and easy separations in...
Keywords: Buffers, Cell disruption, Chromatography, Homogenization, PAGE, Sample preparations, Size-exclusion chromatography, Sonication

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Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
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