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GE17-1194-01 Sigma-Aldrich

HiPrep 26/60 Sephacryl® S-100 HR

GE Healthcare, 17-1194-01, column L × I.D. 60 cm × 26 mm, 50 μm avg. part. size

  •  NACRES SB.52

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Properties

Related Categories Analytical/Chromatography, GE Life Sciences Gel Filtration Columns, Gel Filtration Chromatography, HPLC & FPLC Columns for Biopolymer Separations, HPLC/UHPLC,
material   polypropylene
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
mol wt   10000-100000 Da (globular proteins)
packaging   pkg of 1 ea
mfr. no.   GE Healthcare, 17-1194-01
parameter   <2.6 mL/min flow rate
  0.15 Mpa pressure (22 psi)
bed volume   320 mL
column L × I.D.   60 cm × 26 mm
sample volume   <13 mL
matrix   dextran-acrylamide particle platform
  allyl dextran and N, N’-methylenebisacrylamide
matrix active group   dextran-acrylamide phase
avg. part. size   50 μm
cleaning   2 - 13
working range   3 - 11
separation technique   size exclusion (SEC)

Description

General description

HiPrep 26/60 Sephacryl® S-100 HR are prepacked gel filtration columns for preparative separation of biomolecules from up to 13 mL sample volume. Sephacryl® S-100 is used for separating peptides and small proteins.

Features and Benefits

• Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/bisacrylamide matrix.
• Gel filtration columns for low to medium pressure AKTA systems.
• Well-established in industrial production.
• The HiPrep Sephacryl® series offers reproducible separations over a wide molecular weight range.
• Convenient, prepacked HiPrep Sephacryl® columns speed up protein purification.

Suitability

All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)

Gel filtration columns for low to medium pressure ÄKTA systems.

for preparative separation of biomolecules of up to 13 mL sample volume

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

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Legal Information

HiPrep is a trademark of GE Healthcare

Sephacryl is a registered trademark of GE Healthcare

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Performing a Separation with Sephacryl

Extracted from Size Exclusion Chromatography Principles and Methods, (PDF) GE Healthcare, 2014
Keywords: Buffers, Chromatography, Detergents, Separation

Refolding of proteins using gel filtration

Conceptually, refolding with gel filtration takes advantage of the fact that the gel matrix restricts aggregation through a “cage-like” effect. In addition, the constant removal by the gel filtration m...
Keywords: Dialysis

Separation Options with Sephacryl

Extracted from Size Exclusion Chromatography Principles and Methods, (PDF) GE Healthcare, 2014
Keywords: Chromatography, Fractionation, Separation

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