• USA Home
  • GE17-1194-01 - HiPrep 26/60 Sephacryl® S-100 HR

GE17-1194-01 Sigma-Aldrich

HiPrep 26/60 Sephacryl® S-100 HR

GE Healthcare, 17-1194-01, column L × I.D. 60 cm × 26 mm, 50 μm avg. part. size

  •  NACRES SB.52



Related Categories Analytical/Chromatography, GE Life Sciences Gel Filtration Columns, Gel Filtration Chromatography, HPLC & FPLC Columns for Biopolymer Separations, HPLC/UHPLC,
material   polypropylene
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
mol wt   10000-100000 Da (globular proteins)
packaging   pkg of 1 ea
mfr. no.   GE Healthcare, 17-1194-01
parameter   <2.6 mL/min flow rate
  0.15 Mpa pressure (22 psi)
bed volume   320 mL
column L × I.D.   60 cm × 26 mm
sample volume   <13 mL
matrix   dextran-acrylamide particle platform
  allyl dextran and N, N’-methylenebisacrylamide
matrix active group   dextran-acrylamide phase
avg. part. size   50 μm
cleaning   2 - 13
working range   3 - 11
separation technique   size exclusion (SEC)


General description

HiPrep 26/60 Sephacryl® S-100 HR are prepacked gel filtration columns for preparative separation of biomolecules from up to 13 mL sample volume. Sephacryl® S-100 is used for separating peptides and small proteins.

Features and Benefits

• Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/bisacrylamide matrix.
• Gel filtration columns for low to medium pressure AKTA systems.
• Well-established in industrial production.
• The HiPrep Sephacryl® series offers reproducible separations over a wide molecular weight range.
• Convenient, prepacked HiPrep Sephacryl® columns speed up protein purification.


All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)

Gel filtration columns for low to medium pressure ÄKTA systems.

for preparative separation of biomolecules of up to 13 mL sample volume

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

HiPrep is a trademark of GE Healthcare

Sephacryl is a registered trademark of GE Healthcare

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles


Performing a Separation with Sephacryl

Extracted from Size Exclusion Chromatography Principles and Methods, (PDF) GE Healthcare, 2014
Keywords: Buffers, Chromatography, Detergents, Separation

Refolding of proteins using gel filtration

Conceptually, refolding with gel filtration takes advantage of the fact that the gel matrix restricts aggregation through a “cage-like” effect. In addition, the constant removal by the gel filtration m...
Keywords: Dialysis

Separation Options with Sephacryl

Extracted from Size Exclusion Chromatography Principles and Methods, (PDF) GE Healthcare, 2014
Keywords: Chromatography, Fractionation, Separation

Related Content

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?