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Bromophenol Blue

Cytiva, 17-1329-01, pack of 10 g

  •  NACRES NA.47



Related Categories Molecular Biology, Other Stains for Electrophoresis, Protein Electrophoresis, Protein Stains and Staining Reagents, Proteomics More...
form   solid
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
feature   wetted part: no
packaging   pack of 10 g
mfr. no.   Cytiva, 17-1329-01
storage condition   room temperature
color   Purple, black
pH-range   3.0 - 4.6, yellow to purple
solubility   cold water: soluble (Soluble in cold water and hot water)


General description

PlusOne Bromophenol Blue is an electrophoresis tracking dye used in IEF, PAGE and Sequencing.

Analysis Note


Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

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Protocols & Articles


Components of Rehydration Solution

The choice of the most appropriate rehydration solution for the sample will depend on its specific protein solubility requirements. A typical solution generally contains urea, nonionic or zwitterioni...
Keywords: Buffers, Detergents, Electrophoresis, Isoelectric focusing, Oxidations

Rehydration Solutions

Some of the chemicals used in the procedures—acrylamide, N,N’-methylenebisacrylamide, ammonium persulfate, TEMED, DTT, iodoacetamide, and DeStreak™ Reagent—are very hazardous. Acrylamide monomer, for...
Keywords: Carcinogens, Detergents, Electrophoresis, Isoelectric focusing, Neurotoxins, Separation

Running a Multiphor II Protocol

Ensure that the electrodes in the Immobiline DryStrip tray are connected and place the lid on the Multiphor II unit. Connect the leads on the lid to the power supply. Ensure that the current check on...
Keywords: Electrophoresis, Isoelectric focusing

Troubleshooting First-Dimension IEF: MultiPhor™ II Electrophoresis System and Immobiline® DryStrip Kit

Table 38 lists possible problems that could be encountered during IEF using MultiPhor™ II Electrophoresis System and how to solve them.
Keywords: Electrophoresis, Isoelectric focusing

Troubleshooting Vertical Second Dimension SDS-PAGE

Table 34 lists possible problems that might be encountered during vertical SDS-PAGE and how to solve them.
Keywords: Electrophoresis, PAGE, Polymerization reactions, Separation

Using DeStreak™ Rehydration Solution

Nonspecific oxidation of protein thiol groups is a common problem during 2-D electrophoresis, especially at pH > 7. In the resulting protein map, this problem manifests as horizontal streaks and extr...
Keywords: Buffers, Electrophoresis, Oxidations, Sample preparations

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