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GE17-5068-01 Sigma-Aldrich

SOURCE 15RPC ST 4.6/100

pkg of 1 column

  •  NACRES SB.52

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Properties

Related Categories Analytical/Chromatography, Cytiva Reversed Phase Columns, HPLC & FPLC Columns for Biopolymer Separations, HPLC/UHPLC, Reversed Phase HPLC Columns for Biomolecules More...
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
feature   autoclavable 20 min at 121°C
packaging   pkg of 1 column
parameter   flow rate
  0.5 ml/min - 2.5 ml/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
  580 psi
application(s)   HPLC: suitable
bed size   4.6 mm × 100 mm
bed volume   1.7 mL
column I.D.   4.6 mm
matrix   polystyrene/divinylbenzene
matrix active group   polystyrene-divinylbenzene phase
avg. part. size   15 μm (monosized)
cleaning   1 - 14
working range   1 - 12
capacity   ~10 mg binding capacity(~10 mg BSA /ml medium at 300 cm/h, ~30 mg bacitracin/ml medium at 300 cm/h, ~50 mg insulin/ml medium at 300 cm/h)
suitability   suitable for bioprocess medium
separation technique   reversed phase

Description

General description

SOURCE 15RPC ST 4.6/100 is a prepacked stainless steel column which provides fast, high-capacity preparative protein purification with reversed phase chromatography.

Application

SOURCE 15RPC is based on rigid, monosized 15 μm diameter polystyrene/divinyl benzene beads. The matrix has outstanding selectivity for RPC. The monodispersity of the beads yields stable beds, Low back pressures and excellent results at high flow rates.With its high physical and chemical stability and very high batch-to-batch reproducibility, SOURCE 15RPC is well suited for all stages of an industrial scale operation - from research and process development through scale-up and into production. SOURCE 15RPC offers properties superior to those of other polymeric matrices.

Features and Benefits

• Wide pH range (1-12), outstanding selectivity, chemical resistance, high capacity, and high resolution at high fLow rates.
• Excellent scalability, from RESOURCE to FineLINE columns.
• using ÄKTA® design and other high-performance liquid chromatography systems.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol or 70% Acetonitrile)

Analysis Note

www.gelifesciences.com.

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Legal Information

RESOURCE is a trademark of Cytiva

SOURCE is a trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

Prepacked columns from Cytiva will ensure reproducible results and the highest performance.
Keywords: Antimicrobials, Chromatography, Gene expression, Purification, Reversed-phase chromatography, Separation

Performing an RPC Separation with SOURCE™ Media

Check column performance regularly by determining column efficiency and peak symmetry. See Appendix 2.
Keywords: Buffers, Chromatography, Detergents, Filtration, Reversed-phase chromatography, Separation, Solvents

RPC Purification Options and Scale up with SOURCE™ Media

SOURCE™ media are based on a matrix made from monodispersed, rigid, polystyrene/divinyl benzene (Figure 80). The media demonstrate extreme chemical and physical stability and, unlike silica-based med...
Keywords: Alzheimer Disease, Chromatography, Growth factors, High performance liquid chromatography, Ion Exchange, Liquid chromatography mass spectrometry, Mass spectrometry, Polymerization reactions, Purification, Reversed-phase chromatography, Separation

Reverse Phased Chromatography (RPC) in Practice

As discussed under the theoretical section, a successful RPC separation is influenced by many parameters and will always need to be optimized to meet the requirements for the application. Steps towar...
Keywords: Chromatography, Evaporation, Fractionation, High performance liquid chromatography, Nucleic acid denaturation, PAGE, Phase transitions, Purification, Reversed-phase chromatography, Separation, Solvents, Sonication

Selection of Purifiation Equipment for Hydrophobic Interaction and Reversed Phase Chromatography

A chromatography system is required in order to achieve a high-resolution separation using accurately controlled linear gradient elution, to take advantage of the high flow rates of modern media, or ...
Keywords: Chromatography, Mass spectrometry, Separation

Troubleshooting Reversed Phase Chromatography (RPC)

Poor-quality eluent components can cause a phenomenon referred to as “ghosting”. Trace levels of organic impurities bind to the medium, concentrating during equilibration and sample application. When...
Keywords: Absorption, Chromatography, Evaporation, High performance liquid chromatography, Phase transitions, Precipitation, Reversed-phase chromatography, Sample preparations, Separation

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Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
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