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GE17-5110-01

HiTrap® IGM purification

Cytiva, 17-5110-01, pack of 5 × 1 mL

  •  NACRES NA.56

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Properties

Related Categories Analytical/Chromatography, GE Healthcare Life Sciences Protein Sample Preparation Formats, HiTrap & PD-10 Cartridges, HiTrap System/syringe, Molecular Biology,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 5 × 1 mL
mfr. no.   Cytiva, 17-5110-01
parameter   <4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
  42 psi
bed size   7 mm × 25 mm
bed volume   1 mL
column I.D.   16 mm
matrix   highly cross-linked 6% agarose
avg. part. size   34 μm
cleaning   2 - 13
working range   3 - 11
storage temp.   2-8°C

Description

General description

HiTrap® IgM Purification HP is a 1 mL column prepacked with Sepharose® High Performancefor fast and efficient purification of monoclonal IgM.

Application

HiTrap® IgM Purification HP is prepacked with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose® High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgM and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.

Features and Benefits

• Optimized for fast, efficient purification of monoclonal IgM from hybridoma cell culture.
• Prepacked with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose® High Performance.
• Recovery of IgM is high with excellent activity retention.
• The top and bottom frits are manufactured from porous polyethylene
• Columns are delivered with a stopper on the inlet and a snap-off end on the outle

Analysis Note

www.gelifesciences.com.

Legal Information

HiTrap is a registered trademark of Cytiva

Sepharose is a registered trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
UN1170 - class 3 - PG 3 - Ethanol, solution

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer ...
Keywords: Absorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Electrophoresis, Fractionation, Gel electrophoresis, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Titrations

Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP

HiTrap™ IgM Purification HP 1 ml columns are prepacked with a thiophilic adsorption medium (2-mercaptopyridine coupled to Sepharose® High Performance). The binding capacity of HiTrap™ IgM Purification ...
Keywords: Adsorption, Affinity chromatography, Cell culture, Chromatography, Enzyme-linked immunosorbent assay, Ligands, PAGE, Precipitation, Purification, Sample preparations

Selection Guide for Polymeric Resins in Chromatography & Purification

Sigma-Aldrich carries a vast array of resins, from leading manufacturers; such as, Dow Chemical, Rohm & Haas, Mitsubishi, and Lanxess. Our product line includes adsorbent, ion-exchange (anion / catio...
Keywords: Adsorption, Antibiotics, Combinatorial synthesis, Detergents, Fermentation, Filtration, Hormones, Ion Exchange, Ion exchange resins, Pharmaceutical, Purification, Separation

Protocols

Affinity Chromatography Troubleshooting

This section focuses on practical problems that may occur when running a chromatography column. The diagrams below give an indication of how a chromatogram may deviate from the ideal during affnity p...
Keywords: Adsorption, Affinity chromatography, Buffers, Chromatography, Detergents, Digestions, Fractionation, Ligands, PAGE, Precipitation, Purification, Sample preparations, Separation, Solvents

Buffer Exchange and Desalting for Affinity Chromatography

Dialysis is frequently mentioned in the literature as a technique to remove salt or other small molecules and to exchange the buffer composition of a sample. However, dialysis is generally a very slo...
Keywords: Absorption, Affinity chromatography, Buffers, Chromatography, Dialysis, Separation

HIC Purification Options and Scale up with SOURCE™ Media

SOURCE™ media are based on a hydrophilic matrix made from monodispersed, rigid, polystyrene/divinyl benzene and substituted with hydrophobic ligands: phenyl, ether or isopropyl (Fig 29). The media de...
Keywords: Chromatography, High performance liquid chromatography, Ion Exchange, Ligands, PAGE, Purification, Separation

Performing a Separation with HiTrap IgM Purifcation HP

The technique described here is optimized for purifcation of monoclonal IgM from hybridoma cell culture, but it can be used as a starting point to determine the binding and elution conditions require...
Keywords: Adsorption, Affinity chromatography, Cell culture, Chromatography, Enzyme-linked immunosorbent assay, Ligands, PAGE, Precipitation, Purification

Sample Preparation for Affinity Chromatography

Appendix 1 extracted from Affinity Chromatography Principles and Methods, GE Healthcare, 2007
Keywords: Adsorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Filtration, Nucleic acid denaturation, PAGE, Precipitation, Sample preparations

Selection of Purifiation Equipment for Hydrophobic Interaction and Reversed Phase Chromatography

A chromatography system is required in order to achieve a high-resolution separation using accurately controlled linear gradient elution, to take advantage of the high flow rates of modern media, or ...
Keywords: Chromatography, Mass spectrometry, Separation

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