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GE17-5111-01 Sigma

HiTrap® IGY Purification

GE Healthcare, 17-5111-01, pack of 1 × 5 mL

  •  NACRES NA.56

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Properties

Related Categories Analytical/Chromatography, GE Healthcare Life Sciences Protein Sample Preparation Formats, HiTrap & PD-10 Cartridges, HiTrap System/syringe, Molecular Biology,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 1 × 5 mL
mfr. no.   GE Healthcare, 17-5111-01
parameter   <20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
  42 psi
bed size   16 mm × 25 mm
bed volume   5 mL
column I.D.   16 mm
matrix   highly cross-linked 6% agarose
avg. part. size   34 μm
cleaning   2 - 13
working range   3 - 11
storage temp.   2-8°C

Description

General description

HiTrap® IgY Purification HP is a 5 mL column prepacked with Sepharose® High Performance for fast and easy purification of IgY from egg yolk.

Application

HiTrap® IgY Purification HP is packed with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose® High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgY and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.

Features and Benefits

• Each column binds IgY from a one-quarter portion of egg yolk (approx. 100 mg IgY). For greater capacity, several columns may be connected in series.
• Packed with a thiophilic adsorption medium, the ligand, 2-merCaptopyridine is coupled to Sepharose® High Performance.
• Recovery and purity of IgY are excellent.
• Can be operated with a liquid chromatography system such as ÄKTA® design.
• The top and bottom frits are manufactured from porous polyethylene
• Connectors for usage with different chromatography systems and other equipment are included

Analysis Note

www.gelifesciences.com.

Legal Information

HiTrap is a registered trademark of GE Healthcare

Sepharose is a registered trademark of GE Healthcare

ÄKTA is a registered trademark of GE Healthcare

Safety & Documentation

Safety Information

Symbol 
GHS02  GHS02
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
UN1170 - class 3 - PG 3 - Ethanol, solution

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer ...
Keywords: Absorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Electrophoresis, Fractionation, Gel electrophoresis, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Titrations

Performing a Purification of IgY Antibodies with HiTrap™ IgY Purification HP

HiTrap™ IgY Purification HP 5 ml columns are prepacked with a thiophilic adsorption medium (2-mercaptopyridine coupled to Sepharose® High Performance). The interaction between the protein and the liga...
Keywords: Adsorption, Affinity chromatography, Chromatography, Ligands, PAGE, Precipitation, Purification, Sample preparations

Selection Guide for Polymeric Resins in Chromatography & Purification

Sigma-Aldrich carries a vast array of resins, from leading manufacturers; such as, Dow Chemical, Rohm & Haas, Mitsubishi, and Lanxess. Our product line includes adsorbent, ion-exchange (anion / catio...
Keywords: Adsorption, Antibiotics, Combinatorial synthesis, Detergents, Fermentation, Filtration, Hormones, Ion Exchange, Ion exchange resins, Pharmaceutical, Purification, Separation

Protocols

Affinity Chromatography Troubleshooting

This section focuses on practical problems that may occur when running a chromatography column. The diagrams below give an indication of how a chromatogram may deviate from the ideal during affnity p...
Keywords: Adsorption, Affinity chromatography, Buffers, Chromatography, Detergents, Digestions, Fractionation, Ligands, PAGE, Precipitation, Purification, Sample preparations, Separation, Solvents

Performing a Separation with HiTrap IgY Purifcation HP

HiTrap IgY Purification HP columns are packed with a thiophilic adsorption medium (2-mercaptopyridine coupled to Sepharose High Performance). The interaction between the protein and the ligand has bee...
Keywords: Adsorption, Affinity chromatography, Chromatography, Ligands, PAGE, Precipitation, Purification

Sample Preparation for Affinity Chromatography

Appendix 1 extracted from Affinity Chromatography Principles and Methods, GE Healthcare, 2007
Keywords: Adsorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Filtration, Nucleic acid denaturation, PAGE, Precipitation, Sample preparations

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