• USA Home
  • GE17-5166-01 - Mono Q® 5/50 GL

EMAIL THIS PAGE TO A FRIEND
GE17-5166-01 Sigma-Aldrich

Mono Q® 5/50 GL

  •  NACRES SB.52

Purchase

Properties

Related Categories Analytical/Chromatography, GE Life Sciences Ion Exchange Columns, HPLC & FPLC Columns for Biopolymer Separations, HPLC/UHPLC, Ion Exchange Chromatography,
description   Ion Exchanger Type (value)
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pkg of 1 ea
parameter   0.5 ml/min - 3.0 ml/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
  580 psi
bed volume   1 mL
column I.D.   5.7 mm
matrix   monodisperse porous polystyrene/divinyl benzene beads
matrix active group   ammonium ion, quaternary phase
avg. part. size   10 μm
cleaning   1 - 14
working range   2 - 12
separation technique   anion exchange

Description

General description

Mono Q® is a strong anion exchanger prepacked with MonoBeads in a Tricorncolumn. The column is an excellent choice for small-scale polishing in purification of proteins, peptides, and other biomolecules when high purity is required.

The Tricorn columns offer high-resolution separations with greater loading capacity but slightly lower resolution than MiniBeads columns.

Features and Benefits

• Monodisperse 10 μm porous beads yield high resolution, dynamic capacity, reproducibility, and durability.
• Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q ion exchangers.
• Compatible with AKTA design, FPLC System, and other high-performance LC systems.
• MonoBeads are also available as Mono P® for chromatofocusing

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

FPLC is a trademark of Cytiva

Mono P is a registered trademark of Cytiva

Mono Q is a registered trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Protocols

Affinity Chromatography in a Purification Strategy (CIPP)

Chapter 9, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016
Keywords: Affinity chromatography, Chromatography, Ion Exchange, Ligands, Pharmaceutical, Reversed-phase chromatography, Size-exclusion chromatography, Tagged proteins

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

Appendix 3, extracted from Ion Exchange Chromatography & Chromatofocusing, GE Healthcare, 2007
Keywords: Antimicrobials, Chromatography, Gene expression, Ion Exchange, Purification, Separation

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

Desalting at laboratory scale is a well-proven, simple, and very fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired bu...
Keywords: Absorption, Addition reactions, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Fractionation, Ion Exchange, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Tagged proteins

Ion Exchange Chromatography Troubleshooting

If only certain peaks are of interest in this well-resolved separation, it may be advantageous to transfer to a step elution in order to save time and buffer. The rest of this section focuses on prac...
Keywords: Absorption, Adsorption, Buffers, Chromatography, Detergents, Digestions, Ion Exchange, PAGE, Precipitation, Sample preparations, Separation, Solvents

Monobeads

Use MonoBeads for polishing steps at laboratory scale when highest resolution is essential and a higher capacity than MiniBeads is required.
Keywords: Buffers, Chromatography, Detergents, High performance liquid chromatography, Ion Exchange, PAGE, Precipitation, Purification, Sample preparations, Separation, Solvents, Titrations

Non-volatile and Volatile Buffer Systems

Appendix 2, extracted from Ion Exchange Chromatography & Chromatofocusing, GE Healthcare, 2007
Keywords: Chromatography, Ion Exchange

Optimizing Purification of Histidine-Tagged Proteins

Chapter 4, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Immobilization, Ion Exchange, PAGE, Purification, Separation, Sequencing, Size-exclusion chromatography, Tagged proteins

Performing an isolation of recombinant protein complexes

For structural studies and for comprehensive functional studies of multiprotein complexes it is often necessary to isolate protein amounts in the mg range. As mentioned above, pulldown methods are of...
Keywords: Chromatography, Dialysis, Gene expression, Ion Exchange, Scintillation, Tagged proteins

Practical Considerations for IEX Separation

This section covers detailed aspects of each step in an IEX separation, together with practical hints and tips to improve resolution and overall performance. In practice a separation can be summarize...
Keywords: Absorption, Buffers, Cell culture, Chromatography, Detergents, Filtration, Ion Exchange, Nucleic acid denaturation, PAGE, Phase transitions, Precipitation, Sample preparations, Separation, Solvents

Sample Preparation in Ion Exchange Chromatography

Appendix 1, extracted from Ion Exchange Chromatography & Chromatofocusing, GE Healthcare, 2007
Keywords: Absorption, Adsorption, Affinity chromatography, Amplification, Buffers, Cell culture, Centrifugation, Chromatography, Detergents, Dialysis, Electrophoresis, Filtration, Indicators, Ion Exchange, Nucleic acid denaturation, PAGE, Precipitation, Purification, Sample preparations, Separation

Selection of Purification Equipment

Appendix 4 extracted from Ion Exchange Chromatography & Chromatofocusing, GE Healthcare, 2007
Keywords: Chromatography, Ion Exchange, Purification, Separation

Related Content

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?