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GE17-5177-01 Sigma-Aldrich

Mini Q 4.6/50 PE

  •  NACRES SB.52

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Properties

Related Categories Analytical/Chromatography, Anion Exchange Media - Ion Exchange Chromatography , GE Life Sciences Ion Exchange Columns, HPLC & FPLC Columns for Biopolymer Separations, HPLC/UHPLC,
description   Ion Exchanger Type (value)
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pkg of 1 ea
parameter   2610 psi
bed size   4.6 mm × 50 mm
bed volume   0.8 mL
column I.D.   4.6 mm
matrix   PS-DVB particle platform
  monodisperse polystyrene/divinyl benzene particles
matrix active group   ammonium ion, quaternary phase
particle size   3 μm
cleaning   2 - 14
working range   3 - 11
separation technique   anion exchange

Description

General description

Mini Q 4.6/50 PE columns are prepacked with MiniBeads and are designed for high-performance purification and characterization of biomolecules by ion exchange chromatography. The small particle size of MiniBeads offers the highest resolution.

Precision Column Holder enables use of precision columns with high-performance chromatography systems such as AKTA design.

Features and Benefits

• Monodisperse 3 μm nonporous beads yield exceptional resolution, speed, reproducibility, and durability.
• PC columns are designed for micropurification.
• Nonporous matrix of MiniBeads improves resolution and flow kinetics, better withstands back pressure, and minimizes nonspecific adsorption of the sample.
• Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q and S ion exchangers.
• Nonporous matrix of MiniBeads improves resolution and flow kinetics, better withstands back pressure, and minimizes nonspecific adsorption of the sample.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

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Legal Information

Mini Q is a trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Minibeads

Use MiniBeads for polishing steps at microscale when highest resolution is essential and the capacity of the prepacked column is sufficient.
Keywords: Buffers, Chromatography, Detergents, High performance liquid chromatography, Ion Exchange, PAGE, Precipitation, Purification, Reversed-phase chromatography, Sample preparations, Separation, Solvents

Purification of Membrane Proteins

Extracted from Purifying Challenging Proteins - Principles and Methods, GE Healthcare, 2007
Keywords: Adsorption, Buffers, Cell culture, Cell disruption, Characterizations, Chromatography, Degradations, Detergents, Filtration, Immobilization, Ion Exchange, Ligands, PAGE, Sample preparations, Separation, Sonication, Tagged proteins

Sample Preparation in Ion Exchange Chromatography

Appendix 1, extracted from Ion Exchange Chromatography & Chromatofocusing, GE Healthcare, 2007
Keywords: Absorption, Adsorption, Affinity chromatography, Amplification, Buffers, Cell culture, Centrifugation, Chromatography, Detergents, Dialysis, Electrophoresis, Filtration, Indicators, Ion Exchange, Nucleic acid denaturation, PAGE, Precipitation, Purification, Sample preparations, Separation

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